An application of protein microarray in the screening of monoclonal antibodies against the oyster mushroom spherical virus.

An application of protein microarray in the screening of monoclonal antibodies against the oyster mushroom spherical virus. Research Abstract Details 

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An application of protein microarray in the screening of monoclonal antibodies against the oyster mushroom spherical virus. Abstract Text:

Sang-Woo Kim,Min-Gon Kim,Hyo-Am Jung,Kyung-Hee Lee,Hyun-Sook Lee,Hyeon-Su Ro,

The oyster mushroom spherical virus (OMSV) is a causative agent of dieback disease in the oyster mushroom, Pleurotus ostreatus. Outbreaks of this virus occasionally result in serious disease that is associated with hefty economic losses. Thus, the detection and removal of OMSV-infected spawn is considered to be a crucial step for the stable production of P. ostreatus. For the detection of OMSV, we attempted to generate monoclonal antibodies (mAbs) against an RNA polymerase domain (RPD) of an OMSV protein. In an effort to simplify the laborious multistep mAb screening process, we developed a protein microarray on a slide glass that is chemically modified with the RPD protein. The culture supernatants of 87 hybridoma cells, which were prepared from the fusion of RPD-immunized mouse spleen cells with myeloma cells, were spotted onto the RPD-coated microarray. The binding of mAb to RPD was detected via Alexa 488 dye-labeled anti-mouse immunoglobulin G (IgG) as a secondary antibody. Of 87 samples, 13 evidenced a significant level of fluorescence signal intensity. Subsequent immunoblot analysis revealed that the specificity of each mAb against RPD coincided with the corresponding fluorescence signal intensity, thereby indicating the effectiveness of the protein microarray in mAb screening.

An application of protein microarray in the screening of monoclonal antibodies against the oyster mushroom spherical virus. Publishing Authors By Initials

SW Kim,MG Kim,HA Jung,KH Lee,HS Lee,HS Ro,

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An application of protein microarray in the screening of monoclonal antibodies against the oyster mushroom spherical virus. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical biochemistry

VOLUME: 374

Page Numbers: 313-7

Journal Abbreviation: Anal. Biochem.

ISSN: 0003-2697

DAY: 14

MONTH: 12

YEAR: 2007

An application of protein microarray in the screening of monoclonal antibodies against the oyster mushroom spherical virus. Information

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LANGUAGE: eng

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Grant and Affiliation Information for An application of protein microarray in the screening of monoclonal antibodies against the oyster mushroom spherical virus.

AFFILIATION: Deparment of Microbiology and Research Institute of Life Science, Gyeongsang National University, Chinju 660-701, Korea.

Country: United States

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MEDLINETA: Anal Biochem

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