An annotated cDNA library of juvenile Euprymna scolopes with and without colonization by the symbiont Vibrio fischeri.

An annotated cDNA library of juvenile Euprymna scolopes with and without colonization by the symbiont Vibrio fischeri. Research Abstract Details 

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An annotated cDNA library of juvenile Euprymna scolopes with and without colonization by the symbiont Vibrio fischeri. Abstract Text:

Carlene K Chun,Todd E Scheetz,Maria de Fatima Bonaldo,Bartley Brown,Anik Clemens,Wendy J Crookes-Goodson,Keith Crouch,Tad DeMartini,Mari Eyestone,Michael S Goodson,Bernadette Janssens,Jennifer L Kimbell,Tanya A Koropatnick,Tamara Kucaba,Christina Smith,Jennifer J Stewart,Deyan Tong,Joshua V Troll,Sarahrose Webster,Jane Winhall-Rice,Cory Yap,Thomas L Casavant,Margaret J McFall-Ngai,M Bento Soares,

BACKGROUND: Biologists are becoming increasingly aware that the interaction of animals, including humans, with their coevolved bacterial partners is essential for health. This growing awareness has been a driving force for the development of models for the study of beneficial animal-bacterial interactions. In the squid-vibrio model, symbiotic Vibrio fischeri induce dramatic developmental changes in the light organ of host Euprymna scolopes over the first hours to days of their partnership. We report here the creation of a juvenile light-organ specific EST database. RESULTS: We generated eleven cDNA libraries from the light organ of E. scolopes at developmentally significant time points with and without colonization by V. fischeri. Single pass 3' sequencing efforts generated 42,564 expressed sequence tags (ESTs) of which 35,421 passed our quality criteria and were then clustered via the UIcluster program into 13,962 nonredundant sequences. The cDNA clones representing these nonredundant sequences were sequenced from the 5' end of the vector and 58% of these resulting sequences overlapped significantly with the associated 3' sequence to generate 8,067 contigs with an average sequence length of 1,065 bp. All sequences were annotated with BLASTX (E-value < -03) and Gene Ontology (GO). CONCLUSION: Both the number of ESTs generated from each library and GO categorizations are reflective of the activity state of the light organ during these early stages of symbiosis. Future analyses of the sequences identified in these libraries promise to provide valuable information not only about pathways involved in colonization and early development of the squid light organ, but also about pathways conserved in response to bacterial colonization across the animal kingdom.

An annotated cDNA library of juvenile Euprymna scolopes with and without colonization by the symbiont Vibrio fischeri. Publishing Authors By Initials

CK Chun,TE Scheetz,Mde Bonaldo,B Brown,A Clemens,WJ Crookes-Goodson,K Crouch,T DeMartini,M Eyestone,MS Goodson,B Janssens,JL Kimbell,TA Koropatnick,T Kucaba,C Smith,JJ Stewart,D Tong,JV Troll,S Webster,J Winhall-Rice,C Yap,TL Casavant,MJ McFall-Ngai,MB Soares,

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An annotated cDNA library of juvenile Euprymna scolopes with and without colonization by the symbiont Vibrio fischeri. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: BMC genomics

VOLUME: 7

Page Numbers: 154

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ISSN: 1471-2164

DAY: 16

MONTH: 06

YEAR: 2006

An annotated cDNA library of juvenile Euprymna scolopes with and without colonization by the symbiont Vibrio fischeri. Information

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LANGUAGE: eng

NlmUniqueID: 100965258

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Grant and Affiliation Information for An annotated cDNA library of juvenile Euprymna scolopes with and without colonization by the symbiont Vibrio fischeri.

AFFILIATION: Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706, USA. cchun@wisc.edu

Country: England

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MEDLINETA: BMC Genomics

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