An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material.

An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material. Research Abstract Details 

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An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material. Abstract Text:

D P Mukherjee,A S Tunkle,R A Roberts,A Clavenna,S Rogers,D Smith,

The objective of this study was to develop a synthetic bone graft in a paste form. Reported here are the results of the evaluation of a paste of chitosan glutamate (Protosan) and hydroxyapatite (referred to as a paste) used in a critical size defect model in rats. Eight-millimeter--diameter cranial defects were made in rat calvaria following a protocol approved by the animal review committee. Five groups were studied: (1) empty control, (2) defect filled with paste only, (3) defect filled with the paste containing bone-marrow aspirate, (4) defect filled with paste containing BMP-2, and (5) defect filled with paste containing osteoblasts cultured from bone-marrow aspirate. The sacrifice intervals were 9 and 18 weeks. Calvaria containing the defect were harvested, and the bone mineral density (BMD) was determined by dual energy X-ray absorptiometry. Push-out strength measurements were also performed. The BMD values of empty control were significantly lower than those of other groups at both 9 and 18 weeks. The mechanical properties, that is, push-out strengths and area under the push-out load and displacement were not significantly different between the samples. Histological examination of Goldner-trichromestained undecalcified sections showed the presence of mineralized bone spicules in the defect areas that were more prominent in those filled with paste and osteoblasts cultured from bone-marrow aspirate. Hence, this study demonstrated that the paste of chitosan glutamate and hydroxyapatite-containing osteoblasts cultured from bone-marrow aspirate would be an effective material to repair bone defects.

An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material. Publishing Authors By Initials

DP Mukherjee,AS Tunkle,RA Roberts,A Clavenna,S Rogers,D Smith,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomedical materials research. Part B,

VOLUME: 67

Page Numbers: 603-9

Journal Abbreviation: J. Biomed. Mater. Res. Part B

ISSN: 1552-4973

DAY: 15

MONTH: Oct

YEAR: 2003

An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material. Information

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LANGUAGE: eng

NlmUniqueID: 101234238

An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: pathology

Chemical & Substance for Abstract: An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material. Information

Substance Name: Chitosan

Registry Number: 9012-76-4

Grant and Affiliation Information for An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material.

AFFILIATION: LSU Health Sciences Center, Shreveport, Louisiana 71130-3932, USA. dmukhe@lsuhsc.edu

Country: United States

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MEDLINETA: J Biomed Mater Res B Appl Biom

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