An alcohol oxidase dipstick rapidly detects methanol in the serum of mice.

An alcohol oxidase dipstick rapidly detects methanol in the serum of mice. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

An alcohol oxidase dipstick rapidly detects methanol in the serum of mice. Abstract Text:

Jason B Hack,Jacqueline Early,Kori L Brewer,Jason B Hack,Jacqueline Early,Kori L Brewer,

BACKGROUND: Patients presenting with ingestions of methanol and ethylene glycol pose a significant challenge to emergency physicians. The decision to initiate antidotal therapy must be made quickly and is currently based on the presence of indirect signs, symptoms, and laboratory tests, because no real-time diagnostic test exists to measure these substances. OBJECTIVES: To determine whether a commercially available ethanol-in-saliva detecting dipstick (ALCO-Screen) would be a reliable and rapid indicator of toxic alcohol presence in the serum of an animal model. METHODS: Fifty mice randomly received intraperitoneal doses of methanol, ethylene glycol, or ethanol to induce serum concentrations of approximately 5-400 mg/dL. Thirty minutes after injection, serum was obtained. Serum was both applied to the dipstick and frozen for definitive concentration determination by gas chromatography. After 2 minutes, dipsticks were evaluated for color change by a blinded observer and photographed to be evaluated by other blinded observers at a later time. RESULTS: All concentrations of methanol > or =5 mg/dL consistently caused a color change on the ALCO-Screen. Ethylene glycol reliably caused a color change at > or =300 mg/dL. There was significant agreement among multiple observers whether or not color change had occurred using the ALCO-Screen. CONCLUSIONS: A commercially available dipstick that uses an alcohol oxidase colorimetric reaction reliably and rapidly detects very low serum concentrations of methanol but not ethylene glycol in this animal model. This color change is easily detected by most observers.

An alcohol oxidase dipstick rapidly detects methanol in the serum of mice. Publishing Authors By Initials

JB Hack,J Early,KL Brewer,JB Hack,J Early,KL Brewer,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

An alcohol oxidase dipstick rapidly detects methanol in the serum of mice. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Academic emergency medicine : official journal of

VOLUME: 14

Page Numbers: 1130-4

Journal Abbreviation:

ISSN: 1553-2712

DAY: 29

MONTH: Dec

YEAR: 2007

An alcohol oxidase dipstick rapidly detects methanol in the serum of mice. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9418450

An alcohol oxidase dipstick rapidly detects methanol in the serum of mice. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: An alcohol oxidase dipstick rapidly detects methanol in the serum of mice. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for An alcohol oxidase dipstick rapidly detects methanol in the serum of mice.

AFFILIATION: Department of Emergency Medicine, Brody School of Medicine, East Carolina University, Greenville, NC, USA. hackj@ecu.edu

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Acad Emerg Med

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

An alcohol oxidase dipstick rapidly detects methanol in the serum of mice Related Publications

• A brand new mechanism for copper toxicity.
• A peripatetic pediatrician's journey into pediatric rheumatology: part III.
• An alcohol oxidase dipstick rapidly detects methanol in the serum of mice.
• Analysis of auditory phenotype and karyotype in 200 females with Turner syndrome.
• Association between tetracycline or doxycycline and hepatotoxicity: a population based case-control study.
• Binding studies of alpha-GalNAc-specific lectins to the alpha-GalNAc (Tn-antigen) form of porcine submaxillary mucin and its smaller fragments.
• Brucellosis in general practice.
• Circadian organization of tau mutant hamsters: aftereffects and splitting.
• Competitive binding of AUF1 and TIAR to MYC mRNA controls its translation.
• Coordinate regulation of phospholipid biosynthesis and secretory pathway gene expression in XBP-1(S)-induced endoplasmic reticulum biogenesis.
• Designing lipid nanostructures for local delivery of biologically active macromolecules.
• Diazo preparation via dehydrogenation of hydrazones with "activated" DMSO.
• Director of safe staffing unit can't solve all issues. Interview by Teresa O'Connor.
• Effect of natural antioxidants on oxidative stability of cooked, refrigerated beef and pork.
• Effects of torcetrapib in patients at high risk for coronary events.
• HDL metabolism and the role of HDL in the treatment of high-risk patients with cardiovascular disease.
• In vivo inhibition of E. coli growth by a Ru(II)/Pt(II) supramolecule [(tpy)RuCl(dpp)PtCl2](PF6).
• Local environmental effects and spatial effects in macroecological studies using mapped abundance classes: the case of the rook Corvus frugilegus in Scotland.
• Mapping yeast origins of replication via single-stranded DNA detection.
• Metabolic toxemia of late pregnancy: a disease entity.
• Mitoenergetic failure in Alzheimer disease.
• Part A - microRNAs: discovery, analyses, targets.
• Quality changes in beef gluteus medius, infraspinatus, psoas major, rectus femoris, and teres major enhanced prior to aging.
• Simultaneous age-related depolarization of mitochondrial membrane potential and increased mitochondrial reactive oxygen species production correlate with age-related glutamate excitotoxicity in rat hippocampal neurons.
• The importance of being we: human nature and intergroup relations.
• The independent yet synergistic effects of heparin and dextran.
• The unfolded protein response of B-lymphocytes: PERK-independent development of antibody-secreting cells.
• Tips and techniques for engaging and managing the reluctant, resistant or hostile young person.
• Understanding drug addiction: a neuropsychological perspective.
• Visual field maps in human cortex.