An aerosolized Brucella spp. challenge model for laboratory animals.

An aerosolized Brucella spp. challenge model for laboratory animals. Research Abstract Details 

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An aerosolized Brucella spp. challenge model for laboratory animals. Abstract Text:

S C Olsen,W R Waters,W S Stoffregen,

To characterize the optimal aerosol dosage of Brucella abortus strain 2308 (S2308) and B. melitensis (S16M) in a laboratory animal model of brucellosis, dosages of 10(3)-10(10) colony forming units (CFU) were nebulized to mice. Although tissue weights were minimally influenced, total CFU per tissues increased beginning at 10(6)-10(7) CFU dosages, with 10(9) CFU appearing to be an optimal dosage for S16M or S2308 aerosol delivery. At 12 weeks after vaccination with 10(7) CFU of B. abortus strain RB51 (SRB51) or saline (control), mice were challenged intraperitoneally (i.p.) (6.4 x 10(4) CFU) or via aerosol (1.76 x 10(9) CFU) with S2308. Mice vaccinated with SRB51 had reduced (P < 0.05) splenic, liver and lung colonization (total CFU and CFU/g) after i.p. challenge with S2308 as compared with control mice after i.p. S2308 challenge. Control and SRB51-vaccinated mice did not differ (P > 0.05) in splenic, liver or lung colonization after aerosol S2308 challenge. Failure to demonstrate vaccine protection was not because of a high aerosol challenge dosage as colonization of spleen and liver tissues was lower (P < 0.05) after aerosol challenge when compared with control mice after i.p. S2308 challenge.

An aerosolized Brucella spp. challenge model for laboratory animals. Publishing Authors By Initials

SC Olsen,WR Waters,WS Stoffregen,

For similar bacterial infections and mycoses: zoonoses research abstracts see: bacterial infections and mycoses: zoonoses research

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An aerosolized Brucella spp. challenge model for laboratory animals. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zoonoses and public health

VOLUME: 54

Page Numbers: 281-5

Journal Abbreviation: Zoonoses Public Health

ISSN: 1863-1959

DAY: 27

MONTH: 11

YEAR: 2007

An aerosolized Brucella spp. challenge model for laboratory animals. Information

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LANGUAGE: eng

NlmUniqueID: 101300786

An aerosolized Brucella spp. challenge model for laboratory animals. Keywords Mesh Terms:

KEYWORDS: Zoonoses

MESH TERMS: microbiology

Chemical & Substance for Abstract: An aerosolized Brucella spp. challenge model for laboratory animals. Information

Substance Name: Aerosols

Registry Number: 0

Grant and Affiliation Information for An aerosolized Brucella spp. challenge model for laboratory animals.

AFFILIATION: Bacterial Diseases Research Unit, National Animal Disease Center, USDA, Agriculture, Research Service, Ames, IA 50010, USA. Solsen@NADC.ARS.USDA.GOV

Country: Germany

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MEDLINETA: Zoonoses Public Health

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