An addressable antibody nanoarray produced on a nanostructured surface.

An addressable antibody nanoarray produced on a nanostructured surface. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

An addressable antibody nanoarray produced on a nanostructured surface. Abstract Text:

Andreas Bruckbauer,Dejian Zhou,Dae-Joon Kang,Yuri E Korchev,Chris Abell,David Klenerman,

The ability to address specific nanoscale features is required to produce diverse biological nanoarrays or perform local assembly using biological building blocks and is an important unsolved problem in nanotechnology. In this work, we describe the use of a novel nanofabricated gold surface, with regions of distinct topography and chemical functionalities, to solve this problem. First, nanoarrays of IgG antibodies were produced by selective immobilization in nanoholes on the surface. The smallest feature size was determined by the hole size (fwhm 90 nm) and not surface diffusion. Using holes of 300 nm diameter, we selectively addressed specific features in the array by nanopipet delivery of a functional antibody, anti-IgG. To our knowledge, this is the first example of addressing specific biologically functional features on a surface at the nanoscale.

An addressable antibody nanoarray produced on a nanostructured surface. Publishing Authors By Initials

A Bruckbauer,D Zhou,DJ Kang,YE Korchev,C Abell,D Klenerman,

For similar natural sciences: chemistry: chemistry, physical: surface properties research abstracts see: natural sciences: chemistry: chemistry, physical: surface properties research

PUBMED ID PMID:

MEDLINE DATE:

An addressable antibody nanoarray produced on a nanostructured surface. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 126

Page Numbers: 6508-9

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 2

MONTH: Jun

YEAR: 2004

An addressable antibody nanoarray produced on a nanostructured surface. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

An addressable antibody nanoarray produced on a nanostructured surface. Keywords Mesh Terms:

KEYWORDS: Surface Properties

MESH TERMS: methods

Chemical & Substance for Abstract: An addressable antibody nanoarray produced on a nanostructured surface. Information

Substance Name: Gold

Registry Number: 7440-57-5

Grant and Affiliation Information for An addressable antibody nanoarray produced on a nanostructured surface.

AFFILIATION: Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Am Chem Soc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

An addressable antibody nanoarray produced on a nanostructured surface Related Publications

• A comparison of Wilson and Hohmann osteotomies in the treatment of hallux valgus.
• Absence of the dorsalis pedis pulse in the parents of children with club foot.
• An addressable antibody nanoarray produced on a nanostructured surface.
• Bayesian inference for improved single molecule fluorescence tracking.
• CD161 expression on hepatitis C virus-specific CD8+ T cells suggests a distinct pathway of T cell differentiation.
• CD4+ T cell responses in hepatitis C virus infection.
• Cauda equina and spinal cord compression in Paget's disease.
• Diagnosis of osteomyelitis in neuropathic foot ulcers.
• Diminished frequency of hepatitis C virus specific interferon gamma secreting CD4+ T cells in human immunodeficiency virus/hepatitis C virus coinfected patients.
• Donor and recipient CMV serostatus and antigenemia after renal transplantation: An analysis of 486 patients.
• Dynamics of killer T cell inflation in viral infections.
• Footprints and arches.
• Fracture through the bone of traumatic myositis ossificans. A report of three cases.
• Fractures of the shaft of the humerus.
• Medial calcaneal osteotomy for relapsed equinovarus deformity. Long-term study of the results of Frederick Dwyer.
• Morton's metatarsalgia. Clinical, electrophysiological and histological observations.
• Nanopipette delivery of individual molecules to cellular compartments for single-molecule fluorescence tracking.
• Neuropathic foot ulceration treated by total contact casts.
• Podiatry.
• Quantification and localisation of FOXP3+ T lymphocytes and relation to hepatic inflammation during chronic HCV infection.
• Single-molecule level analysis of the subunit composition of the T cell receptor on live T cells.
• The effect of pneumatic tourniquets on the ultrastructure of skeletal muscle.
• The evolution of the compartment syndrome since 1948 as recorded in the JBJS (B).
• The importance of the toes in walking.
• The management of sprained ankle.
• The management of the infected endoprosthesis.
• The tourniquet in surgery.
• Tourniquet paralysis.
• Two-color fluorescence analysis of individual virions determines the distribution of the copy number of proteins in herpes simplex virus particles.
• Writing with DNA and protein using a nanopipet for controlled delivery.