An activating mutation in the gamma1 subunit of the AMP-activated protein kinase.

An activating mutation in the gamma1 subunit of the AMP-activated protein kinase. Research Abstract Details 

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An activating mutation in the gamma1 subunit of the AMP-activated protein kinase. Abstract Text:

S R Hamilton,D Stapleton,J B O'Donnell,J T Kung,S R Dalal,B E Kemp,L A Witters,

The AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit and two regulatory subunits, beta and gamma. The gamma subunit is essential for enzyme activity by virtue of its binding to the C-terminus of the alpha subunit and appears to play some role in the determination of AMP sensitivity. We demonstrate that a gamma1R70Q mutation causes a marked increase in AMPK activity and renders it largely AMP-independent. This activation is associated with increased phosphorylation of the alpha subunit activation loop T172. These in vitro characteristics of AMPK are also reflected in increased intracellular phosphorylation of one of its major substrates, acetyl-CoA carboxylase. These data illustrate the importance of the gamma1 subunit in the regulation of AMPK and its modulation by AMP.

An activating mutation in the gamma1 subunit of the AMP-activated protein kinase. Publishing Authors By Initials

SR Hamilton,D Stapleton,JB O'Donnell,JT Kung,SR Dalal,BE Kemp,LA Witters,

For similar fungi: yeasts research abstracts see: fungi: yeasts research

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An activating mutation in the gamma1 subunit of the AMP-activated protein kinase. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: FEBS letters

VOLUME: 500

Page Numbers: 163-8

Journal Abbreviation: FEBS Lett.

ISSN: 0014-5793

DAY: 6

MONTH: Jul

YEAR: 2001

An activating mutation in the gamma1 subunit of the AMP-activated protein kinase. Information

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LANGUAGE: eng

NlmUniqueID: 155157

An activating mutation in the gamma1 subunit of the AMP-activated protein kinase. Keywords Mesh Terms:

KEYWORDS: Yeasts

MESH TERMS: genetics

Chemical & Substance for Abstract: An activating mutation in the gamma1 subunit of the AMP-activated protein kinase. Information

Substance Name: AMP-activated protein kinases

Registry Number: EC 2.7.11.31

Grant and Affiliation Information for An activating mutation in the gamma1 subunit of the AMP-activated protein kinase.

AFFILIATION: Endocrine-Metabolism Division, Departments of Medicine and Biochemistry, Dartmouth Medical School, Hanover, NH 03755-3833, USA.

Country: Netherlands

AGENCY: United States NIDDK

GRANT: DK35712

ACRONYM: DK

MEDLINETA: FEBS Lett

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