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An activating factor (tropomyosin) for the superprecipitation of actomyosin prepared from scallop adductor muscles.

An activating factor (tropomyosin) for the superprecipitation of actomyosin prepared from scallop adductor muscles. Research Abstract Details 

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  • An activating factor (tropomyosin) for the superprecipitation of actomyosin prepared from scallop adductor muscles. Abstract Text:

    m takahashiM Takahashi,f moritaF Morita,

    An activating factor for the superprecipitation of actomyosin reconstructed from scallop smooth muscle myosin and rabbit skeletal muscle F-actin was purified from thin filaments of scallop smooth and striated muscles. Two components were obtained from the smooth muscle and one from the striated muscle. All three components similarly affected the actomyosin ATPase activity. According to the results of analysis involving double reciprocal plotting of the ATPase activity versus F-actin concentration, the activating factor for superprecipitation decreased the apparent dissociation constants of actomyosin about 30 to 110 times. The activation of the superprecipitation by the factor, therefore, may be due to the enhancement of the affinity between F-actin and myosin in the presence of ATP. The activating factor was identified as tropomyosin based on it mobility on polyacrylamide gel electrophoresis and on the recovery of the Ca2+-sensitivity of purified rabbit skeletal actomyosin in the presence of troponin.

    An activating factor (tropomyosin) for the superprecipitation of actomyosin prepared from scallop adductor muscles. Publishing Authors By Initials

    m takahashiM Takahashi,f moritaF Morita,

    For similar macromolecular substances: polymers: biopolymers: microfilament proteins: tropomyosin research abstracts see: macromolecular substances: polymers: biopolymers: microfilament proteins: tropomyosin research

    PUBMED ID PMID:

    MEDLINE DATE:

    An activating factor (tropomyosin) for the superprecipitation of actomyosin prepared from scallop adductor muscles. Journal Published:

    PUBLICATION TYPE: Journal Article

    Journal: Journal of biochemistry

    VOLUME: 99

    Page Numbers: 339-47

    Journal Abbreviation: J. Biochem.

    ISSN: 0021-924X

    DAY: 19

    MONTH: Feb

    YEAR: 1986

    An activating factor (tropomyosin) for the superprecipitation of actomyosin prepared from scallop adductor muscles. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 376600

    An activating factor (tropomyosin) for the superprecipitation of actomyosin prepared from scallop adductor muscles. Keywords Mesh Terms:

    KEYWORDS: Tropomyosin

    MESH TERMS: isolation & purification

    Chemical & Substance for Abstract: An activating factor (tropomyosin) for the superprecipitation of actomyosin prepared from scallop adductor muscles. Information

    Substance Name: Adenosine Triphosphatases

    Registry Number: EC 3.6.1.-

    Grant and Affiliation Information for An activating factor (tropomyosin) for the superprecipitation of actomyosin prepared from scallop adductor muscles.

    AFFILIATION:

    Country: JAPAN

    JAPAN Research PublicationJAPAN Research Publication

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    MEDLINETA: J Biochem

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    An activating factor tropomyosin for the superprecipitation of actomyosin prepared from scallop adductor muscles Related Publications

     

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