An accurate and rapid gender determination assay in single cells by the capillary polymerase chain reaction method.

An accurate and rapid gender determination assay in single cells by the capillary polymerase chain reaction method. Research Abstract Details 

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An accurate and rapid gender determination assay in single cells by the capillary polymerase chain reaction method. Abstract Text:

PURPOSE: In preimplantation genetic diagnosis (PGD), a rapid and accurate assay has been required. We have therefore developed a capillary polymerase chain reaction (PCR) method using rapid thermal cycling programs to determine the gender of single amniocytes. METHODS: Single amniocytes from each amniotic fluid sample were isolated by micromanipulation and their gender was determined by a multiplex PCR assay in a capillary tube, using primers that amplify a 308-bp DXZ1 and a 154-bp DYZ1 repeat sequence on the X and Y chromosomes, respectively. RESULTS: All four thermal cycling programs, which took 180, 150, 120, and 90 min, were 100% accurate in diagnosing the gender of single amniocytes. No DNA contamination was observed in any samples. CONCLUSIONS: The multiplex PCR assay was rapid and accurate in diagnosing gender in single cells and may be clinically applicable in PGD.

An accurate and rapid gender determination assay in single cells by the capillary polymerase chain reaction method. Publishing Authors By Initials

For similar genetic processes: sex determination (genetics) research abstracts see: genetic processes: sex determination (genetics) research

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An accurate and rapid gender determination assay in single cells by the capillary polymerase chain reaction method. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of assisted reproduction and genetics

VOLUME: 16

Page Numbers: 551-4

Journal Abbreviation: J. Assist. Reprod. Genet.

ISSN: 1058-0468

DAY: 17

MONTH: Nov

YEAR: 1999

An accurate and rapid gender determination assay in single cells by the capillary polymerase chain reaction method. Information

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LANGUAGE: eng

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An accurate and rapid gender determination assay in single cells by the capillary polymerase chain reaction method. Keywords Mesh Terms:

KEYWORDS: Sex Determination (Genetics)

MESH TERMS: methods

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Grant and Affiliation Information for An accurate and rapid gender determination assay in single cells by the capillary polymerase chain reaction method.

AFFILIATION: Department of Obstetrics and Gynecology, Keio University School of Medicine, Tokyo, Japan.

Country: UNITED STATES

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MEDLINETA: J Assist Reprod Genet

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