Amyloid contained in the knee joint meniscus is formed from apolipoprotein A-I.

Amyloid contained in the knee joint meniscus is formed from apolipoprotein A-I. Research Abstract Details 

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Amyloid contained in the knee joint meniscus is formed from apolipoprotein A-I. Abstract Text:

Alan Solomon,Charles L Murphy,Daniel Kestler,Daniel Coriu,Deborah T Weiss,Josef Makovitzky,Per Westermark,

OBJECTIVE: To determine the chemical nature of amyloid deposits found in knee joint menisci. METHODS: Amyloid was extracted from the menisci of 3 adults who underwent knee joint replacement surgery. The primary structural features of the purified proteins were determined by sequential Edman degradation and tandem mass spectrometry (MS/MS). Tissue specimens were also subjected to in situ hybridization analysis, as well as complementary DNA cloning by reverse transcriptase-polymerase chain reaction (RT-PCR). Additionally, specimens from these 3 patients, as well as other patients with amyloid in the knee joint menisci, were examined immunohistochemically. RESULTS: Amino acid sequence and MS/MS analyses of the extracts revealed the presence of 60-77-residue components identical to the N-terminal portion of apolipoprotein A-I (Apo A-I). The Apo A-I nature of the amyloid was confirmed by the demonstration that the green birefringent congophilic deposits in the 7 meniscus samples were recognized by an anti-human Apo A-I antibody. That the meniscus itself was the source of the amyloidogenic protein was evidenced through Southern blot analysis, in which an Apo A-I product was generated by RT-PCR from synovial tissue, and further, by the demonstration that the cytoplasm of chondrocytes reacted with the specific Apo A-I probe used for in situ hybridization and was immunostained by the anti-Apo A-I antiserum. CONCLUSION: Amyloid in the knee joint menisci is formed from Apo A-I that is produced by chondrocytes within the meniscal cartilage. This entity represents yet another localized form of amyloidosis associated with the aging process and may be of pathophysiologic import.

Amyloid contained in the knee joint meniscus is formed from apolipoprotein A-I. Publishing Authors By Initials

A Solomon,CL Murphy,D Kestler,D Coriu,DT Weiss,J Makovitzky,P Westermark,

For similar proteins: protein precursors research abstracts see: proteins: protein precursors research

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Amyloid contained in the knee joint meniscus is formed from apolipoprotein A-I. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Arthritis and rheumatism

VOLUME: 54

Page Numbers: 3545-50

Journal Abbreviation: Arthritis Rheum.

ISSN: 0004-3591

DAY: 3

MONTH: Nov

YEAR: 2006

Amyloid contained in the knee joint meniscus is formed from apolipoprotein A-I. Information

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LANGUAGE: eng

NlmUniqueID: 370605

Amyloid contained in the knee joint meniscus is formed from apolipoprotein A-I. Keywords Mesh Terms:

KEYWORDS: Protein Precursors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Amyloid contained in the knee joint meniscus is formed from apolipoprotein A-I. Information

Substance Name: Protein Precursors

Registry Number: 0

Grant and Affiliation Information for Amyloid contained in the knee joint meniscus is formed from apolipoprotein A-I.

AFFILIATION: University of Tennessee Center for Health Sciences, College of Medicine, Knoxville, USA. asolomon@mc.utmck.edu

Country: United States

AGENCY: United States NCI

GRANT: CA-10056

ACRONYM: CA

MEDLINETA: Arthritis Rheum

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