Amphotericin B covalent dimers forming sterol-dependent ion-permeable membrane channels.

Amphotericin B covalent dimers forming sterol-dependent ion-permeable membrane channels. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Amphotericin B covalent dimers forming sterol-dependent ion-permeable membrane channels. Abstract Text:

Nobuaki Matsumori,Nahoko Yamaji,Shigeru Matsuoka,Tohru Oishi,Michio Murata,

Polyenemacrolides such as amphotericin B (AmB) were thought to assemble together and form an ion channel across plasma membranes. Their antimicrobial activity has been accounted for by this assemblage, whose stability and activity are dependent on sterol constituents of lipid bilayer membranes. The structure of this channel-like assemblage formed in biomembranes has been a target of extensive investigations for a long time. For the first step to this goal, we prepared several AmB dimers with various linkers and tested for their channel-forming activity. Among these, AmB dimers that bore an aminoalkyl-dicarboxylate tether covalently linked between amino groups of AmB showed potent hemolytic activity. Furthermore, K+ influx actions monitored by measuring the pH of the liposome lumen by 31P NMR revealed that the dimers formed the molecular assemblage similar to that of AmB in phospholipid membrane. Judging from changes in 31P NMR spectra, the dimers appeared to induce "all-or-none"-type ion flux across the liposome membrane in the presence of ergosterol, which suggested that the ion channel formed by ergosterol/dimer is similar to that of AmB. With these data in hand, we are now trying to elucidate the structure of the ion-channel complex by making the labeled conjugates of AmB for NMR measurements.

Amphotericin B covalent dimers forming sterol-dependent ion-permeable membrane channels. Publishing Authors By Initials

N Matsumori,N Yamaji,S Matsuoka,T Oishi,M Murata,

For similar investigative techniques: chemistry, analytical: spectrum analysis: magnetic resonance spectroscopy: nuclear magnetic resonance, biomolecular research abstracts see: investigative techniques: chemistry, analytical: spectrum analysis: magnetic resonance spectroscopy: nuclear magnetic resonance, biomolecular research

PUBMED ID PMID:

MEDLINE DATE:

Amphotericin B covalent dimers forming sterol-dependent ion-permeable membrane channels. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of the American Chemical Society

VOLUME: 124

Page Numbers: 4180-1

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 24

MONTH: Apr

YEAR: 2002

Amphotericin B covalent dimers forming sterol-dependent ion-permeable membrane channels. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Amphotericin B covalent dimers forming sterol-dependent ion-permeable membrane channels. Keywords Mesh Terms:

KEYWORDS: Nuclear Magnetic Resonance, Biomolecular

MESH TERMS: metabolism

Chemical & Substance for Abstract: Amphotericin B covalent dimers forming sterol-dependent ion-permeable membrane channels. Information

Substance Name: Ergosterol

Registry Number: 57-87-4

Grant and Affiliation Information for Amphotericin B covalent dimers forming sterol-dependent ion-permeable membrane channels.

AFFILIATION: Department of Chemistry, Graduate School of Science, Osaka University, 1-16 Machikaneyama, Toyonaka, Osaka 560-0043, Japan.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Am Chem Soc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Amphotericin B covalent dimers forming sterol-dependent ion-permeable membrane channels Related Publications

• A Novel Structural Fold in Polysaccharide Lyases: BACILLUS SUBTILIS FAMILY 11 RHAMNOGALACTURONAN LYASE YesW WITH AN EIGHT-BLADED -PROPELLER.
• A bowl-shaped fullerene encapsulates a water into the cage.
• A case of calcific retropharyngeal tendinitis suspected to be a retropharyngeal abscess upon the first medical examination.
• Amphotericin B covalent dimers forming sterol-dependent ion-permeable membrane channels.
• Automatic regulation of occlusal force because of hardness-change of the bite object.
• Body fluid abnormalities in severe hyperglycemia in patients on chronic dialysis: review of published reports.
• Body fluid abnormalities in severe hyperglycemia in patients on chronic dialysis: theoretical analysis.
• COMPARATIVE KINETICS OF HEMOLYSIS OF MAMMALIAN ERYTHROCYTES BY CLOSTRIDIUM PERFRINGENS ALPHA-TOXIN (PHOSPHOLIPASE C).
• Characterization of protein 4.1R in erythrocytes of zebrafish (Danio rerio): unique binding properties with transmembrane proteins and calmodulin.
• Chemical ionization and electron impact mass spectra of oligosaccharides derived from sphingoglycolipids.
• Conformation and location of membrane-bound salinomycin-sodium complex deduced from NMR in isotropic bicelles.
• Crystal structure of glycoside hydrolase family 78 alpha-L-Rhamnosidase from Bacillus sp. GL1.
• Effect of adenosine 5'-triphosphate and nicotinamide adenine dinucleotide on oxidative phosphorylation of aged brain mitochondria.
• Enzymatic synthesis of p-nitrophenyl alpha-maltopentaoside in an aqueous-methanol solvent system by maltotetraose-forming amylase: a substrate for human amylase in serum.
• Exogenous proline and glycinebetaine increase NaCl-induced ascorbate-glutathione cycle enzyme activities, and proline improves salt tolerance more than glycinebetaine in tobacco Bright Yellow-2 suspension-cultured cells.
• GEL-FILTRATION OF CLOSTRIDIUM PERFRINGENS ALPHA-TOXIN (PHOSPHLIPASE C).
• Involvement of RelA-Associated Inhibitor in Regulation of Trophoblast Differentiation via Interaction with Transcriptional Factor Specificity Protein-1.
• New organic superconductors consisting of an unprecedented pi-electron donor.
• Plant cell wall degradation by saprophytic Bacillus subtilis strains: gene clusters responsible for rhamnogalacturonan depolymerization.
• Preventing effects of a novel anti-parkinsonian agent zonisamide on dopamine quinone formation.
• Reshaping collimated laser beams with Gaussian profile to uniform profiles.
• Rpn10-mediated degradation of ubiquitinated proteins is essential for mouse development.
• Studies on the surface structure of the intracytoplasmic membrane in the photosynthetic purple bacterium Chromatium vinosum by means of chemical modification.
• The essential role of phosphatidylglycerol in photosynthesis.
• Vasoproliferative tumors of the retina secondary to ocular toxocariasis.
• [Comparison of the effects of accelerated heavy ions and X-rays on the development of rat brain]
• [Coronary sinus diverticulum]
• [Effective immunosuppresive therapies including steroid pulse treatment for intramuscular hematoma in iliopsoas in acquired hemophilia]
• [Videofluorographic evaluation of dysphagia in a patient with myasthenia gravis]
• megB1, a novel macroevolutionary genomic marker of the fungal phylum Basidiomycota.