Amphiphysin 1 is important for actin polymerization during phagocytosis.

Amphiphysin 1 is important for actin polymerization during phagocytosis. Research Abstract Details 

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Amphiphysin 1 is important for actin polymerization during phagocytosis. Abstract Text:

Hiroshi Yamada,Emiko Ohashi,Tadashi Abe,Norihiro Kusumi,Shun-Ai Li,Yumi Yoshida,Masami Watanabe,Kazuhito Tomizawa,Yuji Kashiwakura,Hiromi Kumon,Hideki Matsui,Kohji Takei,Hiroshi Yamada,Emiko Ohashi,Tadashi Abe,Norihiro Kusumi,Shun-Ai Li,Yumi Yoshida,Masami Watanabe,Kazuhito Tomizawa,Yuji Kashiwakura,Hiromi Kumon,Hideki Matsui,Kohji Takei,

Amphiphysin 1 is involved in clathrin-mediated endocytosis. In this study, we demonstrate that amphiphysin 1 is essential for cellular phagocytosis and that it is critical for actin polymerization. Phagocytosis in Sertoli cells was induced by stimulating phosphatidylserine receptors. This stimulation led to the formation of actin-rich structures, including ruffles, phagocytic cups, and phagosomes, all of which showed an accumulation of amphiphysin 1. Knocking out amphiphysin 1 by RNA interference in the cells resulted in the reduction of ruffle formation, actin polymerization, and phagocytosis. Phagocytosis was also drastically decreased in amph 1 (-/-) Sertoli cells. In addition, phosphatidylinositol-4,5-bisphosphate-induced actin polymerization was decreased in the knockout testis cytosol. The addition of recombinant amphiphysin 1 to the cytosol restored the polymerization process. Ruffle formation in small interfering RNA-treated cells was recovered by the expression of constitutively active Rac1, suggesting that amphiphysin 1 functions upstream of the protein. These findings support that amphiphysin 1 is important in the regulation of actin dynamics and that it is required for phagocytosis.

Amphiphysin 1 is important for actin polymerization during phagocytosis. Publishing Authors By Initials

H Yamada,E Ohashi,T Abe,N Kusumi,SA Li,Y Yoshida,M Watanabe,K Tomizawa,Y Kashiwakura,H Kumon,H Matsui,K Takei,H Yamada,E Ohashi,T Abe,N Kusumi,SA Li,Y Yoshida,M Watanabe,K Tomizawa,Y Kashiwakura,H Kumon,H Matsui,K Takei,

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Amphiphysin 1 is important for actin polymerization during phagocytosis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Molecular biology of the cell

VOLUME: 18

Page Numbers: 4669-80

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 12

MONTH: 09

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 9201390

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Grant and Affiliation Information for Amphiphysin 1 is important for actin polymerization during phagocytosis.

AFFILIATION: Department of Neuroscience, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan.

Country: United States

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MEDLINETA: Mol Biol Cell

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