Amniotic membrane as support for human retinal pigment epithelium (RPE) cell growth.

Amniotic membrane as support for human retinal pigment epithelium (RPE) cell growth. Research Abstract Details 

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Amniotic membrane as support for human retinal pigment epithelium (RPE) cell growth. Abstract Text:

PURPOSE: The aim of this work was to culture human retinal pigment epithelium (hRPE) cells over human amniotic membrane (hAM). Human AM was studied for its viability as an adequate support for transplantation of an hRPE cell monolayer with preserved cell polarity to the subretinal space. METHODS: Human AM was obtained from pregnant women during caesarean section. The hAM was sectioned and the pieces were fixed to culture dishes. Human RPE cells were cultured from adult corneal donors and were seeded over hAM. Phase-contrast photographs were obtained. Selected specimens were processed by transmission electronic microscopy (TEM). RESULTS: The attachment and growth of hRPE cells over hAM was observed. Human RPE cells constituted tight colonies that maintained epithelial phenotype. Using TEM, we identified a monolayer of hRPE cells, with cuboidal to spheroidal morphology. These cells showed integration with the substrate and cell-cell contacts were detected. CONCLUSION: Amniotic membrane may be a suitable substrate for hRPE growth. Further studies are required in order to determine the viability of hRPE on hAM in the subretinal space.

Amniotic membrane as support for human retinal pigment epithelium (RPE) cell growth. Publishing Authors By Initials

For similar pigment epithelium of eye research abstracts see: pigment epithelium of eye research

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Amniotic membrane as support for human retinal pigment epithelium (RPE) cell growth. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Acta ophthalmologica Scandinavica

VOLUME: 81

Page Numbers: 271-7

Journal Abbreviation: Acta Ophthalmol Scand

ISSN: 1395-3907

DAY: 17

MONTH: Jun

YEAR: 2003

Amniotic membrane as support for human retinal pigment epithelium (RPE) cell growth. Information

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LANGUAGE: eng

NlmUniqueID: 9507578

Amniotic membrane as support for human retinal pigment epithelium (RPE) cell growth. Keywords Mesh Terms:

KEYWORDS: Pigment Epithelium of Eye

MESH TERMS: ultrastructure

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Grant and Affiliation Information for Amniotic membrane as support for human retinal pigment epithelium (RPE) cell growth.

AFFILIATION: Servicio de Oftalmología, Complejo Hospitalario Universitario de Santiago, Santiago de Compostela, Spain.

Country: Denmark

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MEDLINETA: Acta Ophthalmol Scand

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