Ammonium toxicity and potassium limitation in yeast.

Ammonium toxicity and potassium limitation in yeast. Research Abstract Details 

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Ammonium toxicity and potassium limitation in yeast. Abstract Text:

David C Hess,Wenyun Lu,Joshua D Rabinowitz,David Botstein,

DNA microarray analysis of gene expression in steady-state chemostat cultures limited for potassium revealed a surprising connection between potassium and ammonium: potassium limits growth only when ammonium is the nitrogen source. Under potassium limitation, ammonium appears to be toxic for Saccharomyces cerevisiae. This ammonium toxicity, which appears to occur by leakage of ammonium through potassium channels, is recapitulated under high-potassium conditions by over-expression of ammonium transporters. Although ammonium toxicity is well established in metazoans, it has never been reported for yeast. To characterize the response to ammonium toxicity, we examined the filtrates of these cultures for compounds whose excretion might serve to detoxify the ammonium (such as urea in mammals). Using liquid chromatography-tandem mass spectrometry to assay for a wide array of metabolites, we detected excreted amino acids. The amounts of amino acids excreted increased in relation to the severity of growth impairment by ammonium, suggesting that amino acid excretion is used by yeast for ammonium detoxification.

Ammonium toxicity and potassium limitation in yeast. Publishing Authors By Initials

DC Hess,W Lu,JD Rabinowitz,D Botstein,

For similar fungi: yeasts research abstracts see: fungi: yeasts research

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Ammonium toxicity and potassium limitation in yeast. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: PLoS biology

VOLUME: 4

Page Numbers: e351

Journal Abbreviation: PLoS Biol.

ISSN: 1545-7885

DAY: 3

MONTH: Oct

YEAR: 2006

Ammonium toxicity and potassium limitation in yeast. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101183755

Ammonium toxicity and potassium limitation in yeast. Keywords Mesh Terms:

KEYWORDS: Yeasts

MESH TERMS: physiology

Chemical & Substance for Abstract: Ammonium toxicity and potassium limitation in yeast. Information

Substance Name: Ammonia

Registry Number: 7664-41-7

Grant and Affiliation Information for Ammonium toxicity and potassium limitation in yeast.

AFFILIATION: Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA. dhess@princeton.edu

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM046406

ACRONYM: GM

MEDLINETA: PLoS Biol

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