Amino acid substitutions in the -35 recognition motif of sigma 70 that result in defects in phage lambda repressor-stimulated transcription.

Amino acid substitutions in the -35 recognition motif of sigma 70 that result in defects in phage lambda repressor-stimulated transcription. Research Abstract Details 

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Amino acid substitutions in the -35 recognition motif of sigma 70 that result in defects in phage lambda repressor-stimulated transcription. Abstract Text:

The phage lambda repressor activates transcription of its own gene from the promoter PRM. Previous work has suggested that this activation involves a protein-protein interaction between DNA-bound repressor and RNA polymerase. To identify the subunit of RNA polymerase that participates in this putative interaction, we searched for polymerase mutants that responded poorly to repressor. We report here the isolation of three sigma mutants that caused defects in repressor-stimulated, but not basal, transcription from PRM. These mutants bear amino acid substitutions in a putative helix-turn-helix motif that sigma uses to recognize the promoter -35 region. We suggest that lambda repressor interacts directly with this helix-turn-helix motif in facilitating the formation of a productive initiating complex.

Amino acid substitutions in the -35 recognition motif of sigma 70 that result in defects in phage lambda repressor-stimulated transcription. Publishing Authors By Initials

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Amino acid substitutions in the -35 recognition motif of sigma 70 that result in defects in phage lambda repressor-stimulated transcription. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of bacteriology

VOLUME: 176

Page Numbers: 2991-8

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 14

MONTH: May

YEAR: 1994

Amino acid substitutions in the -35 recognition motif of sigma 70 that result in defects in phage lambda repressor-stimulated transcription. Information

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LANGUAGE: eng

NlmUniqueID: 2985120

Amino acid substitutions in the -35 recognition motif of sigma 70 that result in defects in phage lambda repressor-stimulated transcription. Keywords Mesh Terms:

KEYWORDS: beta-Galactosidase

MESH TERMS: genetics

Chemical & Substance for Abstract: Amino acid substitutions in the -35 recognition motif of sigma 70 that result in defects in phage lambda repressor-stimulated transcription. Information

Substance Name: beta-Galactosidase

Registry Number: EC 3.2.1.23

Grant and Affiliation Information for Amino acid substitutions in the -35 recognition motif of sigma 70 that result in defects in phage lambda repressor-stimulated transcription.

AFFILIATION: Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.

Country: UNITED STATES

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MEDLINETA: J Bacteriol

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