Amino acid residues in Rag1 crucial for DNA hairpin formation.

Amino acid residues in Rag1 crucial for DNA hairpin formation. Research Abstract Details 

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Amino acid residues in Rag1 crucial for DNA hairpin formation. Abstract Text:

Catherine P Lu,Hector Sandoval,Vicky L Brandt,Phoebe A Rice,David B Roth,

The Rag proteins carry out V(D)J recombination through a process mechanistically similar to cut-and-paste transposition. Specifically, Rag complexes form DNA hairpins through direct transesterification, using a catalytic Asp-Asp-Glu (DDE) triad in Rag1. How is sufficient DNA distortion introduced to allow hairpin formation? We hypothesized that, like certain transposases, the Rag proteins might use aromatic amino acid residues to stabilize a flipped-out base. Through in vivo and in vitro experiments and structural predictions, we identified residues in Rag1 crucial for hairpin formation. One of these, a conserved tryptophan (Trp893), probably participates in base-stacking interactions near the cleavage site, as do Trp298, Trp265 and Trp319 in the Tn5, Tn10 and Hermes transposases, respectively. Other residues surrounding the catalytic glutamate (YKEFRK) may share functional similarities with the YREK motif in IS4 family transposases.

Amino acid residues in Rag1 crucial for DNA hairpin formation. Publishing Authors By Initials

CP Lu,H Sandoval,VL Brandt,PA Rice,DB Roth,

For similar enzymes and coenzymes: enzymes: recombinases: vdj recombinases research abstracts see: enzymes and coenzymes: enzymes: recombinases: vdj recombinases research

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Amino acid residues in Rag1 crucial for DNA hairpin formation. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature structural & molecular biology

VOLUME: 13

Page Numbers: 1010-5

Journal Abbreviation: Nat. Struct. Mol. Biol.

ISSN: 1545-9993

DAY: 8

MONTH: 10

YEAR: 2006

Amino acid residues in Rag1 crucial for DNA hairpin formation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101186374

Amino acid residues in Rag1 crucial for DNA hairpin formation. Keywords Mesh Terms:

KEYWORDS: VDJ Recombinases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Amino acid residues in Rag1 crucial for DNA hairpin formation. Information

Substance Name: VDJ Recombinases

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Amino acid residues in Rag1 crucial for DNA hairpin formation.

AFFILIATION: Program in Molecular Pathogenesis, Skirball Institute of Biomolecular Medicine, and Department of Pathology, New York University School of Medicine, New York, New York 10016, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI36420

ACRONYM: AI

MEDLINETA: Nat Struct Mol Biol

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