Amino acid, ammonia and urea concentrations in human pre-ovulatory ovarian follicular fluid.

Amino acid, ammonia and urea concentrations in human pre-ovulatory ovarian follicular fluid. Research Abstract Details 

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Amino acid, ammonia and urea concentrations in human pre-ovulatory ovarian follicular fluid. Abstract Text:

Marcin ,Maciej ,Cecilia Teng,Frederick C Battaglia,

BACKGROUND: This study aimed to determine amino acid (AA), ammonia and urea concentrations in human ovarian follicular fluid and to compare these concentrations with those in the circulation. METHODS: Samples of pre-ovulatory follicular fluid and peripheral venous blood were obtained from 14 IVF patients. High-performance liquid chromatography (HPLC) measurements of 25 AAs were the main outcome measures. RESULTS: There was a significant gradient of most AAs from plasma to follicular fluid, with the exception of glutamate, which demonstrated a three-fold increase in follicular fluid concentration (70.0 +/- 3.80 microM) compared with plasma (23.18 +/- 2.20 microM; P < 0.001). The plasma-to-follicular fluid concentration difference for glutamine (81.83 +/- 9.2 microM) was greatest among all AAs. Among essential AAs, this difference was greatest for the branched-chain AAs, isoleucine, leucine and valine. Ammonia concentrations in follicular fluid and blood were 38.87 +/- 2.23 and 22.11 +/- 1.96 microM, respectively (P < 0.001). Urea concentration in follicular fluid was 3.37 +/- 0.18 mM, a value not significantly different from plasma concentration (3.36 +/- 0.22 mM; P = 0.911). CONCLUSIONS: These plasma-follicular fluid differences may reflect both the utilization of AAs and the transport characteristics of the follicular cells. There is accumulation of glutamate and ammonia in pre-ovulatory follicular fluid. The data for urea are consistent with transport by passive diffusion, with no evidence of an active urea cycle in the cells of the follicle.

Amino acid, ammonia and urea concentrations in human pre-ovulatory ovarian follicular fluid. Publishing Authors By Initials

M ,M ,C Teng,FC Battaglia,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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Amino acid, ammonia and urea concentrations in human pre-ovulatory ovarian follicular fluid. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Human reproduction (Oxford, England)

VOLUME: 21

Page Numbers: 2776-82

Journal Abbreviation: Hum. Reprod.

ISSN: 0268-1161

DAY: 1

MONTH: 09

YEAR: 2006

Amino acid, ammonia and urea concentrations in human pre-ovulatory ovarian follicular fluid. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8701199

Amino acid, ammonia and urea concentrations in human pre-ovulatory ovarian follicular fluid. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: analysis

Chemical & Substance for Abstract: Amino acid, ammonia and urea concentrations in human pre-ovulatory ovarian follicular fluid. Information

Substance Name: Ammonia

Registry Number: 7664-41-7

Grant and Affiliation Information for Amino acid, ammonia and urea concentrations in human pre-ovulatory ovarian follicular fluid.

AFFILIATION: Department of Gynecology, Medical University of Bialystok, Bialystok, Poland. jozwik@obgyn.edu.pl

Country: England

AGENCY: United States NCRR

GRANT: M01 RR000069

ACRONYM: RR

MEDLINETA: Hum Reprod

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