Amination and subsequent derivatization of epoxy-activated agarose for the preparation of new affinity adsorbents.

Amination and subsequent derivatization of epoxy-activated agarose for the preparation of new affinity adsorbents. Research Abstract Details 

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Amination and subsequent derivatization of epoxy-activated agarose for the preparation of new affinity adsorbents. Abstract Text:

I Matsumoto,N Seno,A M Golovtchenko-Matsumoto,T Osawa,

We have found a simple procedure to convert epoxy-activated agarose into amino derivatives using ammonia solution. The amino derivatives of agarose were succinylated with succinic anhydride and then activated with N-hydroxysuccinimide according to the method of Cuatrecasas and Parikh. Formula: (See Text). Coupling of the ligand to the activated agarose (shown above) was performed under mild conditions. The adsorbent thus obtained has no charged group in the linkage region between the ligand and agarose, thus reducing the nonspecific adsorption, and the bonds formed (ether and amide bonds) are stable even in an alkaline medium. Lens culinaris hemagglutinin-Sepharose 4B prepared by this method was successfully used for the affinity chromatography of solubilized human red blood cell membrane in detergent solution and was stable when elution was performed with borate buffer, pH 9.8.

Amination and subsequent derivatization of epoxy-activated agarose for the preparation of new affinity adsorbents. Publishing Authors By Initials

I Matsumoto,N Seno,AM Golovtchenko-Matsumoto,T Osawa,

For similar carbohydrates: polysaccharides: sepharose research abstracts see: carbohydrates: polysaccharides: sepharose research

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Amination and subsequent derivatization of epoxy-activated agarose for the preparation of new affinity adsorbents. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 87

Page Numbers: 535-40

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1980

Amination and subsequent derivatization of epoxy-activated agarose for the preparation of new affinity adsorbents. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Amination and subsequent derivatization of epoxy-activated agarose for the preparation of new affinity adsorbents. Keywords Mesh Terms:

KEYWORDS: Sepharose

MESH TERMS: analysis

Chemical & Substance for Abstract: Amination and subsequent derivatization of epoxy-activated agarose for the preparation of new affinity adsorbents. Information

Substance Name: Sepharose

Registry Number: 9012-36-6

Grant and Affiliation Information for Amination and subsequent derivatization of epoxy-activated agarose for the preparation of new affinity adsorbents.

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Country: JAPAN

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MEDLINETA: J Biochem

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