Amidolytic activity of porcine trypsin bound to human plasma alpha-1-antiproteinase.

Amidolytic activity of porcine trypsin bound to human plasma alpha-1-antiproteinase. Research Abstract Details 

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Amidolytic activity of porcine trypsin bound to human plasma alpha-1-antiproteinase. Abstract Text:

A Saito,H Sinohara,

Human plasma alpha-1-antiproteinase interacted with porcine trypsin in two different manners. One was a well known interaction, which resulted in inhibition of the proteolytic activity of the trypsin. The other has not been described to date, and resulted in retention of the amidolytic activity of the trypsin towards benzoyl-L-arginine p-nitroanilide in the presence of soybean trypsin inhibitor. The latter, so-called trypsin-protein amidase, activity is essentially the same as that observed with vertebrate alpha-macroglobulin and rodent murinoglobulin under similar conditions. All attempts to separate the two different activities as well as to abolish either activity by means of chemical or physical modifications were unsuccessful. The proteolysis-inhibiting interaction, which was virtually completed within 5 min, was predominant over the amidolysis-retaining interaction, when the inhibitor/trypsin molar ratio was less than 1. On the other hand, the amidolysis-retaining interaction, which proceeded much more slowly, became evident when the molar ratio was greater than 1.

Amidolytic activity of porcine trypsin bound to human plasma alpha-1-antiproteinase. Publishing Authors By Initials

A Saito,H Sinohara,

For similar peptides: proteinase inhibitory proteins, secretory: alpha 1-antitrypsin research abstracts see: peptides: proteinase inhibitory proteins, secretory: alpha 1-antitrypsin research

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Amidolytic activity of porcine trypsin bound to human plasma alpha-1-antiproteinase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 103

Page Numbers: 254-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Feb

YEAR: 1988

Amidolytic activity of porcine trypsin bound to human plasma alpha-1-antiproteinase. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Amidolytic activity of porcine trypsin bound to human plasma alpha-1-antiproteinase. Keywords Mesh Terms:

KEYWORDS: alpha 1-Antitrypsin

MESH TERMS: metabolism

Chemical & Substance for Abstract: Amidolytic activity of porcine trypsin bound to human plasma alpha-1-antiproteinase. Information

Substance Name: Amidohydrolases

Registry Number: EC 3.5.-

Grant and Affiliation Information for Amidolytic activity of porcine trypsin bound to human plasma alpha-1-antiproteinase.

AFFILIATION: Department of Biochemistry, Kinki University School of Medicine, Osaka.

Country: JAPAN

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MEDLINETA: J Biochem

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