Alternatives to chromatographic separations.

Alternatives to chromatographic separations. Research Abstract Details 

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Alternatives to chromatographic separations. Abstract Text:

,Mark Etzel,

Up to now, the productivity of mammalian cell culture has been perceived as limiting the productivity of the industrial manufacture of therapeutic monoclonal antibodies. Dramatic improvements in cell culture performance have changed this picture, and the throughput of antibody purification processes is gaining increasing attention. Although chromatographic separations currently are the centerpiece of antibody purification, mostly due to their high resolving power, it becomes more and more apparent that there may be limitations at the very large scale. This review will discuss a number of alternatives to chromatographic antibody purification, with a particular emphasis on the ability to increase throughput and overcome traditional drawbacks of column chromatography. Specifically, precipitation, membrane chromatography, high-resolution ultrafiltration, crystallization, and high-pressure refolding will be evaluated as potential large scale unit operations for industrial antibody production.

Alternatives to chromatographic separations. Publishing Authors By Initials

J ,M Etzel,

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PUBMED ID PMID:

MEDLINE DATE: 2007 Jan-Feb

Alternatives to chromatographic separations. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biotechnology progress

VOLUME: 23

Page Numbers: 42-5

Journal Abbreviation: Biotechnol. Prog.

ISSN: 8756-7938

DAY: 2

MONTH: 02

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 8506292

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Grant and Affiliation Information for Alternatives to chromatographic separations.

AFFILIATION: Biogen IDEC, Inc., 5200 Research Place, San Diego, California 92122, and University of Wisconsin, 1605 Linden Drive, Madison, Wisconsin 53706.

Country: United States

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MEDLINETA: Biotechnol Prog

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