Alternative splicing produces a constitutively active form of human SREBP-1.

Alternative splicing produces a constitutively active form of human SREBP-1. Research Abstract Details 

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Alternative splicing produces a constitutively active form of human SREBP-1. Abstract Text:

Nagakatsu Harada,Haruka Yonemoto,Masaki Yoshida,Hironori Yamamoto,Yunjie Yin,Aiko Miyamoto,Atsushi Hattori,Qishisan Wu,Tadahiko Nakagawa,Masayuki Nakano,Kiyoshi Teshigawara,Kazuaki Mawatari,Toshio Hosaka,Akira Takahashi,Yutaka Nakaya,

We identified a novel alternative splicing event that constitutively produces a truncated active form of human sterol regulatory element-binding protein 1 (SREBP-1). A cDNA of this splicing variant (named SREBP-1Delta) contains a translational stop codon-encoding exon sequence between exons 7 and 8. It produces SREBP-1aDelta (470 a.a.) and SREBP-1cDelta (446 a.a.) proteins that lack transmembrane and C-terminal regulatory sequences necessary for localization of SREBP-1 to the endoplasmic reticulum. A luciferase reporter assay showed that SREBP-1aDelta and SREBP-1cDelta transactivated lipogenic gene promoters to the same extent as that induced by N-terminal active fragments of SREBP-1a and SREBP-1c, respectively. SREBP-1Delta mRNA is expressed in human cell lines as well as adipose and liver tissues. Expression levels ranged from 5% to 16% of total SREBP-1 expression. The ratio of SREBP-1Delta expression to total SREBP-1 expression in HepG2 cells was not affected by either insulin or high glucose treatment.

Alternative splicing produces a constitutively active form of human SREBP-1. Publishing Authors By Initials

N Harada,H Yonemoto,M Yoshida,H Yamamoto,Y Yin,A Miyamoto,A Hattori,Q Wu,T Nakagawa,M Nakano,K Teshigawara,K Mawatari,T Hosaka,A Takahashi,Y Nakaya,

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Alternative splicing produces a constitutively active form of human SREBP-1. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biochemical and biophysical research communication

VOLUME: 368

Page Numbers: 820-6

Journal Abbreviation: Biochem. Biophys. Res. Commun.

ISSN: 1090-2104

DAY: 11

MONTH: 02

YEAR: 2008

Alternative splicing produces a constitutively active form of human SREBP-1. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Alternative splicing produces a constitutively active form of human SREBP-1.

AFFILIATION: Department of Nutrition and Metabolism, Institute of Health Biosciences, University of Tokushima Graduate School, 3-18-15, Kuramoto-cho, Tokushima City 770-8503, Japan.

Country: United States

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MEDLINETA: Biochem Biophys Res Commun

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