Altered whisker patterns induced by ectopic expression of Shh are topographically represented by barrels.

Altered whisker patterns induced by ectopic expression of Shh are topographically represented by barrels. Research Abstract Details 

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Altered whisker patterns induced by ectopic expression of Shh are topographically represented by barrels. Abstract Text:

Kanae Ohsaki,Noriko Osumi,Shun Nakamura,

Barrels in the somatosensory cortex are segregated columns, which somatotopically relate to facial whiskers. The barrel pattern is assumed to be determined by an extrinsic mechanism (the domino theory). This theory is based on whisker lesion experiments and developmental observations regarding the serial establishment of the somatotopic pattern in which pattern formations are relayed from the periphery to the central nervous system. However, the barrel pattern is possibly determined by an intrinsic mechanism, especially in its primitive form. In order to investigate the definitive mechanism, we established an experimental system in which the cortical barrel pattern can be altered, not by using a lesion paradigm, but by epigenetically changing the whisker pattern. Sonic hedgehog (Shh) plays a pivotal role in whisker development. We transfected an adenovirus harboring chicken Shh (Ad-cShh) to mouse embryos (E9.5-E11.5) using an in utero surgical technique. When Ad-cShh was expressed in the epidermis, Bmp4, Ptch, Ptch2 and Gli1 were induced ectopically in the interfollicular region. In contrast, the expression of Bmp2 and Shh itself was unaltered. At a suitable dose of Ad-cShh, some pups displayed supernumerary whiskers or a disordered whisker pattern. The barrel patterns of these mice after the critical period were topographic representations of the contralateral side of the new whisker patterns when visualized by a cytochrome oxidase or Nissle staining method, supporting the instructive role of the extrinsic mechanism.

Altered whisker patterns induced by ectopic expression of Shh are topographically represented by barrels. Publishing Authors By Initials

K Ohsaki,N Osumi,S Nakamura,

For similar animal structures: vibrissae research abstracts see: animal structures: vibrissae research

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Altered whisker patterns induced by ectopic expression of Shh are topographically represented by barrels. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Brain research. Developmental brain research

VOLUME: 137

Page Numbers: 159-70

Journal Abbreviation: Brain Res. Dev. Brain Res.

ISSN: 0165-3806

DAY: 30

MONTH: Aug

YEAR: 2002

Altered whisker patterns induced by ectopic expression of Shh are topographically represented by barrels. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8908639

Altered whisker patterns induced by ectopic expression of Shh are topographically represented by barrels. Keywords Mesh Terms:

KEYWORDS: Vibrissae

MESH TERMS: metabolism

Chemical & Substance for Abstract: Altered whisker patterns induced by ectopic expression of Shh are topographically represented by barrels. Information

Substance Name: patched receptors

Registry Number: 0

Grant and Affiliation Information for Altered whisker patterns induced by ectopic expression of Shh are topographically represented by barrels.

AFFILIATION: Division of Biochemistry and Cellular Biology, National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi, Kodaira, 187-8502, Tokyo, Japan.

Country: Netherlands

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MEDLINETA: Brain Res Dev Brain Res

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