Altered protein expression at early-stage rat hepatic neoplasia.

Altered protein expression at early-stage rat hepatic neoplasia. Research Abstract Details 

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Altered protein expression at early-stage rat hepatic neoplasia. Abstract Text:

Qilie Luo,Linda Siconolfi-Baez,Pallavi Annamaneni,Mark T Bielawski,Phyllis M Novikoff,Ruth Hogue Angeletti,

Protein expression patterns were analyzed in a rat model of hepatic neoplasia to detect changes reflecting biological mechanism or potential therapeutic targets. The rat resistant hepatocyte model of carcinogenesis was studied, with a focus on the earliest preneoplastic lesion visible in the liver, the preneoplastic hyperplastic nodule. Expression differences were shown by two-dimensional polyacrylamide gel electrophoresis and image analysis. Polypeptide masses were measured by peptide mass fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF) and their sequences were obtained by tandem mass spectrometry. Alterations in expression of cytoskeletal and functional proteins were demonstrated, consistent with biological changes known to occur in the preneoplastic cells. Of particular interest was the differential expression of a serine protease inhibitor (serpin) with a role implicated in angiogenesis. Serpin, implicated in the inhibition of angiogenesis, is present in normal liver but has greatly reduced expression at the preneoplastic stage of liver cancer development. Immunofluorescence microscopy with antibodies to this serpin, kallistatin, supports the proteomic identification. Immunofluorescence microscopy with antibodies to the blood vessel marker von Willebrand factor provides evidence for neovascularization in the liver containing multiple preneoplastic nodules. These observations suggest that at an early stage of liver carcinogenesis reduction or loss of angiogenesis inhibitors may contribute to initiation of neoangiogenesis. A number of other identified proteins known to be associated with hepatomas are also present at early-stage neoplasia.

Altered protein expression at early-stage rat hepatic neoplasia. Publishing Authors By Initials

Q Luo,L Siconolfi-Baez,P Annamaneni,MT Bielawski,PM Novikoff,RH Angeletti,

For similar proteins: serpins research abstracts see: proteins: serpins research

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Altered protein expression at early-stage rat hepatic neoplasia. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: American journal of physiology. Gastrointestinal a

VOLUME: 292

Page Numbers: G1272-82

Journal Abbreviation: Am. J. Physiol. Gastrointest.

ISSN: 0193-1857

DAY: 1

MONTH: 02

YEAR: 2007

Altered protein expression at early-stage rat hepatic neoplasia. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100901227

Altered protein expression at early-stage rat hepatic neoplasia. Keywords Mesh Terms:

KEYWORDS: Serpins

MESH TERMS: analysis

Chemical & Substance for Abstract: Altered protein expression at early-stage rat hepatic neoplasia. Information

Substance Name: Serpins

Registry Number: 0

Grant and Affiliation Information for Altered protein expression at early-stage rat hepatic neoplasia.

AFFILIATION: Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York , USA.

Country: United States

AGENCY: United States PHS

GRANT: P30 KD-41296

ACRONYM: CA

MEDLINETA: Am J Physiol Gastrointest Live

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