Altered mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas.

Altered mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas. Research Abstract Details 

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Altered mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas. Abstract Text:

K T Lee,T S Liu,

Neoplastic transformation of epithelial cells is commonly associated with alterations in the synthesis and structures of mucin. Mucin protein epitopes and mRNA levels were frequently altered in adenocarcinomas compared to corresponding normal tissues. Clinically, hepatolithiasis has been regarded as a risk factor for cholangiocarcinoma. The aims of this study were to determine the possible alteration of mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas and to try to predict whether or not hepatolithiasis has a predisposition to development of cholangiocarcinoma. In situ hybridization with DIG-tailed oligonucleotides was performed on sections of paraffin-embedded tissues of stone-containing intrahepatic bile ducts, cholangiocarcinomas, and normal controls to identify the expression of MUC2, MUC3, MUC4, MUC5B, and MUC5AC in nonneoplastic and neoplastic biliary epithelium. The findings showed that (1) while multiple diverse mucin genes were expressed in the biliary epithelium, MUC3 and MUC5B mRNA were the main mucin genes expressed in the biliary epithelium of stone-containing intrahepatic bile ducts and normal controls; (2) absent or decreased expression of MUC2, MUC3, and MUC5B of mRNA was found in cholangiocarcinomas in contrast to nonneoplastic biliary epithelium; and (3) increased expression of MUC4 and MU5AC of mRNA was found in cholangiocarcinomas and the biliary epithelium, especially for dysplastic cells of stone-containing intrahepatic bile ducts compared with normal controls. In this study, using in situ hybridization we demonstrated that neoplastic transformation of the biliary epithelium is accompained by alterations in mucin gene expression, the altered mucin gene expression in dysplastic cells of stone-containing intrahepatic bile ducts may reflect a higher potential for malignant transformation in these cells, and it could be a precursor of cholangiocarcinoma in the presence of hepatolithiasis.

Altered mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas. Publishing Authors By Initials

KT Lee,TS Liu,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, neoplasm research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, neoplasm research

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Altered mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Digestive diseases and sciences

VOLUME: 46

Page Numbers: 2166-72

Journal Abbreviation: Dig. Dis. Sci.

ISSN: 0163-2116

DAY: 1

MONTH: Oct

YEAR: 2001

Altered mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas. Information

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LANGUAGE: eng

NlmUniqueID: 7902782

Altered mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas. Keywords Mesh Terms:

KEYWORDS: RNA, Neoplasm

MESH TERMS: metabolism

Chemical & Substance for Abstract: Altered mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas. Information

Substance Name: mucin 5AC

Registry Number: 0

Grant and Affiliation Information for Altered mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas.

AFFILIATION: Department of Surgery, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.

Country: United States

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MEDLINETA: Dig Dis Sci

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