Altered extracellular matrix expression in the diverted fetal sheep bladder.

Altered extracellular matrix expression in the diverted fetal sheep bladder. Research Abstract Details 

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Altered extracellular matrix expression in the diverted fetal sheep bladder. Abstract Text:

Wenjie Wei,Pamela S Howard,Barry Kogan,Edward J Macarak,

PURPOSE: It is unclear whether filling and emptying are important to bladder development. We tested this in an experimental preparation. MATERIAL AND METHODS: Urinary diversion was performed in 7 fetal lambs at 90 days of gestation and 6 unoperated fetal lambs served as controls. Transmural sections were analyzed for changes in tissue layer thickness and/or composition after 14 days of urinary diversion. Matrix mRNA levels (collagen I and III, and FN) as well as the cytokines/growth factors IGF-1, EGR-1, WT-1 and BCL-2 were quantified by real-time polymerase chain reaction. Hydroxyproline measurements of total collagen and collagen subtype quantification were done by enzyme-linked immunosorbent assay. RESULTS: Diverted fetal bladders showed a 27% and 57% decrease in mucosal and detrusor muscle layer thickness, respectively. In contrast, there was a 270% increase in serosal layer thickness in diverted bladders. The mRNA levels of COL1A1, COL3A1, IGF-1, EGR-1 and the anti-apoptotic gene BCL-2 were increased significantly in the serosal/detrusor layer of diverted bladders. In the mucosa levels of these mRNAs remained unchanged except for those of FN and WT-1, which were significantly decreased and increased, respectively. Total collagen, and type I and III collagen protein levels were significantly increased in diverted bladders. CONCLUSIONS: The lack of mechanical loading in diverted bladders leads to the arrest of detrusor smooth muscle growth, and concurrent fibrosis and thickening of the serosal layer. Changes in the levels of IGF-1, BCL-2 and EGR-1 likely have regulatory roles that affect the smooth muscle phenotype in the detrusor layer.

Altered extracellular matrix expression in the diverted fetal sheep bladder. Publishing Authors By Initials

W Wei,PS Howard,B Kogan,EJ Macarak,

For similar proteins: neoplasm proteins: tumor suppressor proteins: wt1 proteins research abstracts see: proteins: neoplasm proteins: tumor suppressor proteins: wt1 proteins research

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Altered extracellular matrix expression in the diverted fetal sheep bladder. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of urology

VOLUME: 178

Page Numbers: 1104-7

Journal Abbreviation: J. Urol.

ISSN: 0022-5347

DAY: 20

MONTH: 07

YEAR: 2007

Altered extracellular matrix expression in the diverted fetal sheep bladder. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376374

Altered extracellular matrix expression in the diverted fetal sheep bladder. Keywords Mesh Terms:

KEYWORDS: WT1 Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Altered extracellular matrix expression in the diverted fetal sheep bladder. Information

Substance Name: Collagen

Registry Number: 9007-34-5

Grant and Affiliation Information for Altered extracellular matrix expression in the diverted fetal sheep bladder.

AFFILIATION: Department of Anatomy and Cell Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK54987

ACRONYM: DK

MEDLINETA: J Urol

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