Alterations of platelet function induced by interleukin-2.

Alterations of platelet function induced by interleukin-2. Research Abstract Details 

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Alterations of platelet function induced by interleukin-2. Abstract Text:

We recently reported that thrombocytopenia and bleeding are often limiting effects of immunotherapy with interleukin-2 (IL-2). In order to understand the mechanisms that lead to this unexpected clinical toxicity, we studied the effects of IL-2 on in vitro platelet function. When platelet aggregation was studied using whole blood (impedance, electrical) aggregometry, inhibition of aggregation was detected within 1 min of the addition of exogenous IL-2 to whole blood. IL-2-induced platelet secretion was quantified by radioimmunoassay (RIA) of PF4, BTG, and TXB2 independent of the addition of an aggregating agonist (ADP). Platelet secretion and inhibition of aggregation were an indirect consequence of a cellular effect of IL-2 on mononuclear cells, since aggregation was normal when whole blood was depleted of mononuclear cells and its reconstitution with autologous mononuclear cells led to a cell concentration-dependent inhibitory effect of aggregation and release of alpha-granule components in the presence of IL-2. In order to understand the mechanism of platelet secretion mediated by IL-2-activated mononuclear cells, we quantified the release of eicosanoid products from cultures of mononuclear cells exposed to IL-2 and found a significant increase in TXB2. Our results indicate that platelet secretion, indirectly initiated by IL-2-activated cells, is followed by inhibition of aggregation. These findings may not only have important implications for the planning of clinical immunotherapy trials with IL-2, but may also provide a novel link for a better understanding of the relationships between the hemostatic and the immune systems.

Alterations of platelet function induced by interleukin-2. Publishing Authors By Initials

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

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Alterations of platelet function induced by interleukin-2. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of immunotherapy : official journal of the

VOLUME: 10

Page Numbers: 363-70

Journal Abbreviation: J. Immunother.

ISSN: 1053-8550

DAY: 18

MONTH: Oct

YEAR: 1991

Alterations of platelet function induced by interleukin-2. Information

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LANGUAGE: eng

NlmUniqueID: 9102704

Alterations of platelet function induced by interleukin-2. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Alterations of platelet function induced by interleukin-2. Information

Substance Name: Recombinant Proteins

Registry Number: 0

Grant and Affiliation Information for Alterations of platelet function induced by interleukin-2.

AFFILIATION: Jeanne B. Lambert Laboratory for Cancer Research, Department of Neoplastic Diseases, Mount Sinai Medical Center, New York, NY 10029.

Country: UNITED STATES

AGENCY: United States NCI

GRANT: 5-P30-CA23102

ACRONYM: CA

MEDLINETA: J Immunother (1991)

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