Alteration of the protein kinase binding domain enhances function of the Saccharomyces cerevisiae molecular chaperone Cdc37.

Alteration of the protein kinase binding domain enhances function of the Saccharomyces cerevisiae molecular chaperone Cdc37. Research Abstract Details 

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Alteration of the protein kinase binding domain enhances function of the Saccharomyces cerevisiae molecular chaperone Cdc37. Abstract Text:

Min Ren,Arti Santhanam,Paul Lee,Avrom Caplan,Stephen Garrett,

Cdc37 is a molecular chaperone that has a general function in the biogenesis of protein kinases. We identified mutations within the putative "protein kinase binding domain" of Cdc37 that alleviate the conditional growth defect of a strain containing a temperature-sensitive allele, tpk2(Ts), of the cyclic AMP-dependent protein kinase (PKA). These dominant mutations alleviate the temperature-sensitive growth defect by elevating PKA activity, as judged by their effects on PKA-regulated processes, localization and phosphorylation of the PKA effector Msn2, as well as in vitro PKA activity. Although the tpk2(Ts) growth defect is also alleviated by Cdc37 overproduction, the CDC37 dominant mutants contain wild-type Cdc37 protein levels. In addition, Saccharomyces cerevisiae Ste11 protein kinase has an elevated physical interaction with the altered Cdc37 protein. These results implicate specific amino-terminal residues in the interaction between Cdc37 and client protein kinases and provide further genetic and biochemical support for a model in which Cdc37 functions as a molecular chaperone for protein kinases.

Alteration of the protein kinase binding domain enhances function of the Saccharomyces cerevisiae molecular chaperone Cdc37. Publishing Authors By Initials

M Ren,A Santhanam,P Lee,A Caplan,S Garrett,

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Alteration of the protein kinase binding domain enhances function of the Saccharomyces cerevisiae molecular chaperone Cdc37. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Eukaryotic cell

VOLUME: 6

Page Numbers: 1363-72

Journal Abbreviation: Eukaryotic Cell

ISSN: 1535-9778

DAY: 15

MONTH: 06

YEAR: 2007

Alteration of the protein kinase binding domain enhances function of the Saccharomyces cerevisiae molecular chaperone Cdc37. Information

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LANGUAGE: eng

NlmUniqueID: 101130731

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Grant and Affiliation Information for Alteration of the protein kinase binding domain enhances function of the Saccharomyces cerevisiae molecular chaperone Cdc37.

AFFILIATION: Graduate School of Biomedical Sciences, Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, 225 Warren St., Newark, NJ 07101, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM70596

ACRONYM: GM

MEDLINETA: Eukaryot Cell

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