Alpha1A-adrenergic receptors are functionally expressed by a subpopulation of cornu ammonis 1 interneurons in rat hippocampus.

Alpha1A-adrenergic receptors are functionally expressed by a subpopulation of cornu ammonis 1 interneurons in rat hippocampus. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Alpha1A-adrenergic receptors are functionally expressed by a subpopulation of cornu ammonis 1 interneurons in rat hippocampus. Abstract Text:

Kristin L Hillman,Van A Doze,James E Porter,

The importance of adrenergic receptors (ARs) in the hippocampus has generally focused on betaARs; however, interest is growing in hippocampal alphaARs given their purported neuroprotective role. We have previously reported alpha(1)AR transcripts in a subpopulation of cornu ammonis 1 (CA1) interneurons. The goal of this study was to identify the specific alpha(1)AR subtype (alpha(1A), alpha(1B), alpha(1D)) functionally expressed by these cells. Using cell-attached recordings to measure action potential frequency changes, concentration-response curves for the selective alpha(1)AR agonist phenylephrine (PE) were generated in the presence of competitive subtype-selective alpha(1)AR antagonists. Schild regression analysis was then used to estimate equilibrium dissociation constants (K(b)) for each receptor antagonist in our system. The selective alpha(1A)AR antagonists, 5-methylurapidil and WB-4101 [2-[(2,6-dimethoxyphenoxyethyl)aminomethyl]-1,4-benzodioxane hydrochloride], produced consecutive rightward shifts in the concentration-response curve for PE when used at discriminating, nanomolar concentrations. Calculated K(b) values for 5-methylurapidil (10 nM) and WB-4101 (5 nM) correlate to previously published affinity values for these antagonists at the alpha(1A)AR. The selective alpha(1B)AR antagonist L-765,314 [(2S)-4-(4-amino-6,7-dimethoxy-2-quinazolinyl)-2-[[(1,1-dimethylethyl)amino]carbonyl]-1-piperazinecarboxylic acid], as well as the selective alpha(1D)AR antagonist BMY7378 [8-[2-[4-(2-methoxyphenyl)-1-piperazinyl]ethyl]-8-azaspiro[4.5]decane-7,9-dione dihydrochloride], produced significant rightward shifts in the concentration-response curve for PE only when used at nondistinguishing, micromolar concentrations. Calculated K(b) values for L-765,314 (794 nM) and BMY7378 (316 nM) do not agree with affinity values for these antagonists at the alpha(1B) or alpha(1D)AR, respectively. Rather, these K(b) values more closely match equilibrium dissociation constants estimated for these compounds when used to identify alpha(1A)AR subtypes. Together, our results provide strong evidence to support functional expression of alpha(1A)ARs in a subpopulation of CA1 interneurons.

Alpha1A-adrenergic receptors are functionally expressed by a subpopulation of cornu ammonis 1 interneurons in rat hippocampus. Publishing Authors By Initials

KL Hillman,VA Doze,JE Porter,

For similar proteins: membrane proteins: receptors, cell surface: receptors, biogenic amine: receptors, catecholamine: receptors, adrenergic: receptors, adrenergic, alpha: receptors, adrenergic, alpha-1 research abstracts see: proteins: membrane proteins: receptors, cell surface: receptors, biogenic amine: receptors, catecholamine: receptors, adrenergic: receptors, adrenergic, alpha: receptors, adrenergic, alpha-1 research

PUBMED ID PMID:

MEDLINE DATE:

Alpha1A-adrenergic receptors are functionally expressed by a subpopulation of cornu ammonis 1 interneurons in rat hippocampus. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of pharmacology and experimental thera

VOLUME: 321

Page Numbers: 1062-8

Journal Abbreviation: J. Pharmacol. Exp. Ther.

ISSN: 0022-3565

DAY: 2

MONTH: 03

YEAR: 2007

Alpha1A-adrenergic receptors are functionally expressed by a subpopulation of cornu ammonis 1 interneurons in rat hippocampus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376362

Alpha1A-adrenergic receptors are functionally expressed by a subpopulation of cornu ammonis 1 interneurons in rat hippocampus. Keywords Mesh Terms:

KEYWORDS: Receptors, Adrenergic, alpha-1

MESH TERMS: physiology

Chemical & Substance for Abstract: Alpha1A-adrenergic receptors are functionally expressed by a subpopulation of cornu ammonis 1 interneurons in rat hippocampus. Information

Substance Name: Isoproterenol

Registry Number: 7683-59-2

Grant and Affiliation Information for Alpha1A-adrenergic receptors are functionally expressed by a subpopulation of cornu ammonis 1 interneurons in rat hippocampus.

AFFILIATION: Department of Pharmacology, Physiology, and Therapeutics, University of North Dakota School of Medicine and Health Sciences, 501 North Columbia Road, Stop 9037, Grand Forks, North Dakota 58202-9037, USA.

Country: United States

AGENCY: United States NCRR

GRANT: 5P20RR017699

ACRONYM: RR

MEDLINETA: J Pharmacol Exp Ther

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Alpha1A-adrenergic receptors are functionally expressed by a subpopulation of cornu ammonis 1 interneurons in rat hippocampus Related Publications

• 1157.
• Actions taken by frail older widows to allow home care providers access to their homes.
• Alpha1A-adrenergic receptors are functionally expressed by a subpopulation of cornu ammonis 1 interneurons in rat hippocampus.
• Chemical and statistical considerations in the determination of partition coefficients of weakly ionizable drugs and poisons.
• Commentary.
• Discovery and SAR studies of novel GlyT1 inhibitors.
• Do interventions to increase income improve the health of the poor in developed economies and are such policies cost effective?
• Enhancing Short-Term RecoverY after High Intensity Anaerobic Exercise: 1835: Board #123 May 31 9:00 AM 10:30 AM.
• Examination of the role of intestinal fatty acid-binding protein in drug absorption using a parallel artificial membrane permeability assay.
• Extranodal NK/T-cell lymphoma, nasal type.
• GLOBAL INITIATIVE The Economic Case.
• Genetically altered expression of spermidine/spermine N1-acetyltransferase affects fat metabolism in mice via acetyl-CoA.
• Group II metabotropic glutamate receptors inhibit glutamate release at thalamocortical synapses in the developing somatosensory cortex.
• Hepatic cytochrome P450 reductase-null mice reveal a second microsomal reductase for squalene monooxygenase.
• In vivo and in vitro lipid peroxidation of arachidonate esters: the effect of fish oil omega-3 lipids on product distribution.
• Inhibition of breast cancer growth and invasion by single-minded 2s.
• Measurement of driving patterns of older adults using data logging devices with and without global positioning system capability.
• Mechanism of TNFalpha-induced IL-1alpha, IL-1beta and IL-6 expression in human cardiac fibroblasts: effects of statins and thiazolidinediones.
• Phospholipid-protein adducts of lipid peroxidation: synthesis and study of new biotinylated phosphatidylcholines.
• Polyamine metabolism and tumorigenesis in the Apc(Min/+) mouse.
• Postoperative radiographs following hip fracture surgery. Do they influence patient management?
• Predicting diabetic ketoacidosis in children by measuring end-tidal CO2 via non-invasive nasal capnography.
• Regression of myelofibrosis and osteosclerosis following hematopoietic cell transplantation assessed by magnetic resonance imaging and histologic grading.
• Target practice. In search engine marketing, opportunity outweighs risk.
• Tetrahydro-1,8-naphthyridinol analogues of alpha-tocopherol as antioxidants in lipid membranes and low-density lipoproteins.
• The medial surgical approach to the humerus.
• The unclear role of ethnicity in health inequalities: the scenario of oral cancer incidence and survival in the British South Asian population.
• Treatment of upper tract urothelial carcinoma: a review of surgical and adjuvant therapy.
• Use of Global Positioning System (GPS) Watches and Accelerometry to Monitor Mobility in Older Adults: 628: May 30 4:30 PM 4:45 PM.
• Validity, trustworthiness and rigour: reasserting realism in qualitative research.