Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham's F-10.

Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham's F-10. Research Abstract Details 

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Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham's F-10. Abstract Text:

PROBLEM: The development of mouse embryos in vitro is affected primarily by mouse strain-genotype and culture conditions. Embryo culture studies evaluate the effectiveness of culture conditions in supporting one- or two-cell mouse embryo development to the blastocyst stage by reporting the percentage blastocyst formation rate. METHOD: Determining the cell number per cultured blastocyst may also help in determining embryo culture medium quality. The objective of this study was to determine the effect of MEM and Ham's F-10 on overall CFW mouse embryo development and hatched blastocyst cell number in vitro. CFW embryos cultured in MEM had significantly higher (87%; P < 0.001) hatched blastocyst rates than embryos cultured in F-10 (56%). RESULTS: A significant difference in nuclei per hatched blastocyst was found between MEM and F-10 (P < 0.001). The results demonstrate that inbred mouse embryos have significantly higher blastocyst hatching rates and higher cell numbers per blastocyst when cultured in MEM.

Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham's F-10. Publishing Authors By Initials

For similar cells: trophoblasts research abstracts see: cells: trophoblasts research

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Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham's F-10. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of assisted reproduction and genetics

VOLUME: 11

Page Numbers: 203-7

Journal Abbreviation: J. Assist. Reprod. Genet.

ISSN: 1058-0468

DAY: 17

MONTH: Apr

YEAR: 1994

Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham's F-10. Information

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LANGUAGE: eng

NlmUniqueID: 9206495

Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham's F-10. Keywords Mesh Terms:

KEYWORDS: Trophoblasts

MESH TERMS: cytology

Chemical & Substance for Abstract: Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham's F-10. Information

Substance Name: Culture Media

Registry Number: 0

Grant and Affiliation Information for Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham's F-10.

AFFILIATION: Department of Obstetrics and Gynecology, Medical University of South Carolina, Charleston 29425-2233.

Country: UNITED STATES

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MEDLINETA: J Assist Reprod Genet

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