Alpha-linolenic acid attenuates high glucose-induced apoptosis in cultured human umbilical vein endothelial cells via PI3K/Akt/eNOS pathway.

Alpha-linolenic acid attenuates high glucose-induced apoptosis in cultured human umbilical vein endothelial cells via PI3K/Akt/eNOS pathway. Research Abstract Details 

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Alpha-linolenic acid attenuates high glucose-induced apoptosis in cultured human umbilical vein endothelial cells via PI3K/Akt/eNOS pathway. Abstract Text:

Wei Zhang,Rui Wang,Shu-fang Han,Lun Bu,Si-wang Wang,Heng Ma,Guo-liang Jia,

OBJECTIVE: High glucose-induced apoptosis in vascular endothelial cells contributes to the acceleration of atherosclerosis associated with diabetes. We hypothesized that alpha-linolenic acid (ALA) might attenuate high glucose-induced apoptosis in cultured human umbilical vein endothelial cells (HUVECs). METHODS: HUVECs were cultured at 5.5 and 33 mmol/L for 72 h. ALA with different concentrations was added with defatted bovine serum albumin as a carrier for 18 h before incubation with high glucose. RESULTS: Exposure of HUVECs to high glucose media for 72 h significantly increased the number of apoptotic cells compared with normal glucose control, as evaluated by flow cytometry and terminal deoxyuridine triphosphate nick end labeling assay. Pretreatment with low concentrations of ALA (10, 50, and 100 micromol/L) significantly attenuated high glucose-induced apoptosis of HUVECs, but increasing ALA to 200 micromol/L exerted the opposite effect. Furthermore, high glucose reduced phosphorylation of Akt and endothelial nitric oxide synthase (eNOS) with subsequent nitric oxide production, whereas ALA treatment attenuated the reduction caused by high glucose. Pretreatment with phosphatidylinositol 3' -kinase kinase inhibitor LY294002 and eNOS inhibitor N(G)-nitro-arginine methyl ester eliminated ALA' antiapoptotic effect. CONCLUSION: ALA exerts an antiapoptotic effect by the phosphatidylinositol 3'-kinase/Akt/eNOS pathway in HUVECs exposed to high glucose and thus may represent a candidate therapeutic agent for diabetic cardiovascular complications.

Alpha-linolenic acid attenuates high glucose-induced apoptosis in cultured human umbilical vein endothelial cells via PI3K/Akt/eNOS pathway. Publishing Authors By Initials

W Zhang,R Wang,SF Han,L Bu,SW Wang,H Ma,GL Jia,

For similar lipids: fats: dietary fats: dietary fats, unsaturated: fatty acids, omega-3: alpha-linolenic acid research abstracts see: lipids: fats: dietary fats: dietary fats, unsaturated: fatty acids, omega-3: alpha-linolenic acid research

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Alpha-linolenic acid attenuates high glucose-induced apoptosis in cultured human umbilical vein endothelial cells via PI3K/Akt/eNOS pathway. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Nutrition (Burbank, Los Angeles County, Calif.)

VOLUME: 23

Page Numbers: 762-70

Journal Abbreviation:

ISSN: 0899-9007

DAY: 23

MONTH: 08

YEAR: 2007

Alpha-linolenic acid attenuates high glucose-induced apoptosis in cultured human umbilical vein endothelial cells via PI3K/Akt/eNOS pathway. Information

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LANGUAGE: eng

NlmUniqueID: 8802712

Alpha-linolenic acid attenuates high glucose-induced apoptosis in cultured human umbilical vein endothelial cells via PI3K/Akt/eNOS pathway. Keywords Mesh Terms:

KEYWORDS: alpha-Linolenic Acid

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Alpha-linolenic acid attenuates high glucose-induced apoptosis in cultured human umbilical vein endothelial cells via PI3K/Akt/eNOS pathway. Information

Substance Name: Oncogene Protein v-akt

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Alpha-linolenic acid attenuates high glucose-induced apoptosis in cultured human umbilical vein endothelial cells via PI3K/Akt/eNOS pathway.

AFFILIATION: Department of Cardiology, Tangdu hospital, Fourth Military Medical University, Xi'an, People's Republic of China. zhangwei@fmmu.edu.cn

Country: United States

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MEDLINETA: Nutrition

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