Allogeneic cultured dermal substitute composed of spongy collagen containing fibroblasts: evaluation in animal test.

Allogeneic cultured dermal substitute composed of spongy collagen containing fibroblasts: evaluation in animal test. Research Abstract Details 

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Allogeneic cultured dermal substitute composed of spongy collagen containing fibroblasts: evaluation in animal test. Abstract Text:

M Tanaka,N Nakakita,Y Kuroyanagi,

The authors developed a cultured dermal substitute (CDS) composed of a spongy collagen containing cultured fibroblasts. The cultured fibroblasts derived from Sprague Dawley rat skin were seeded on a spongy collagen at a density of 5 x 10(5) cells cm(-2) and cultured for 7 days. This CDS was applied to the debrided wound of full-thickness burn which was inflicted experimentally on the dorsum of Wister rat, and then the wound conditions were observed over a period of 2 weeks. The comparative study was conducted using an acellular spongy collagen as well as a commercially available temporary wound dressing, Biobrane, since a different type of cultured dermal substitute, Dermagraft-TC, is composed of Biobrane, whose inner site is combined with cultured fibroblasts. Each covering material was applied on the debrided wound area and exchanged by new one 1 week later. When the debrided wound was covered with Biobrane, a small portion of necrotic tissue was observed 1 week after application, and the granulation tissue formation was greatly delayed. This wound area showed a poor granulation tissue even 2 weeks later. On the contrary, when covered with an acellular spongy collagen, no necrotic tissue was observed. This wound area showed a more or less irregular granulation tissue at 1 week and then a healthy granulation tissue 2 weeks later. This preliminary comparative study suggests that an acellular spongy collagen is able to function as a more suitable matrix for CDS, compared with Biobrane. The wound area covered with a CDS assumed a moist, shiny, and hyperaemic appearance 1 week after application showing a healthy granulation tissue. The macroscopic evaluations indicate that the CDS is able to prepare a healthy granulation tissue at an earlier stage, compared with the acellular spongy collagen. In addition, the histologic views demonstrate that the CDS is able to prepare a thicker and denser granulation tissue, compared with the acellular spongy collagen. Although the fate of cultured fibroblasts in the CDS on the wound surface within 1 week is not clear, these findings suggest that fibroblasts in CDS are able to provide excellent conditions for wound healing.

Allogeneic cultured dermal substitute composed of spongy collagen containing fibroblasts: evaluation in animal test. Publishing Authors By Initials

M Tanaka,N Nakakita,Y Kuroyanagi,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: regeneration: wound healing research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: regeneration: wound healing research

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Allogeneic cultured dermal substitute composed of spongy collagen containing fibroblasts: evaluation in animal test. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biomaterials science. Polymer edition

VOLUME: 10

Page Numbers: 433-53

Journal Abbreviation:

ISSN: 0920-5063

DAY: 20

MONTH: 02

YEAR: 1999

Allogeneic cultured dermal substitute composed of spongy collagen containing fibroblasts: evaluation in animal test. Information

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LANGUAGE: eng

NlmUniqueID: 9007393

Allogeneic cultured dermal substitute composed of spongy collagen containing fibroblasts: evaluation in animal test. Keywords Mesh Terms:

KEYWORDS: Wound Healing

MESH TERMS: pathology

Chemical & Substance for Abstract: Allogeneic cultured dermal substitute composed of spongy collagen containing fibroblasts: evaluation in animal test. Information

Substance Name: Collagen

Registry Number: 9007-34-5

Grant and Affiliation Information for Allogeneic cultured dermal substitute composed of spongy collagen containing fibroblasts: evaluation in animal test.

AFFILIATION: Department of Plastic and Reconstructive Surgery, School of Medicine, Kitasato University, Sagamihara, Kanagawa, Japan.

Country: NETHERLANDS

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MEDLINETA: J Biomater Sci Polym Ed

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