Allocation of gamma-tubulin between oocyte cortex and meiotic spindle influences asymmetric cytokinesis in the mouse oocyte.

Allocation of gamma-tubulin between oocyte cortex and meiotic spindle influences asymmetric cytokinesis in the mouse oocyte. Research Abstract Details 

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Allocation of gamma-tubulin between oocyte cortex and meiotic spindle influences asymmetric cytokinesis in the mouse oocyte. Abstract Text:

Susan L Barrett,David F Albertini,

In oocytes, asymmetric cytokinesis represents a conserved strategy for karyokinesis during meiosis to retain ooplasmic maternal factors needed after fertilization. Given the role of gamma-tubulin in cell cycle progression and microtubule dynamics, this study focused on gamma-tubulin as a key regulator of asymmetric cytokinesis in mouse oocytes. Gamma-tubulin properties were studied using multiple-label digital imaging, Western blots, quantitative RT-PCR, and microinjection strategies in mouse oocytes matured in vivo (IVO) or in vitro (IVM). Quantitative image analysis established that IVO oocytes extrude smaller first polar bodies (PBs), contain smaller spindles, and have more cytoplasmic microtubule organizing centers (MTOCs) relative to IVM oocytes. Maturation in culture was shown to alter gamma-tubulin distribution, as evidenced by incorporation throughout the meiotic spindle and within the first PB. Western blot analysis confirmed that total gamma-tubulin content remained elevated in IVM oocytes compared with IVO oocytes. Analysis of gamma-tubulin mRNA during maturation revealed fluctuations in IVO oocytes, whereas IVM oocytes maintained relatively stable at lower levels for the time points examined (0-16 h). Selective reduction of gamma-tubulin mRNA by injection of siRNA diminished both spindle and PB size, whereas overexpression of enhanced green fluorescent protein gamma-tubulin had the opposite effect. Together, these studies reinforce the notion that limiting gamma-tubulin availability during meiotic maturation ensures coordination of karyokinesis and cytokinesis and conservation of gamma-tubulin as an embryonic reserve.

Allocation of gamma-tubulin between oocyte cortex and meiotic spindle influences asymmetric cytokinesis in the mouse oocyte. Publishing Authors By Initials

SL Barrett,DF Albertini,

For similar macromolecular substances: polymers: biopolymers: microtubule proteins: tubulin research abstracts see: macromolecular substances: polymers: biopolymers: microtubule proteins: tubulin research

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Allocation of gamma-tubulin between oocyte cortex and meiotic spindle influences asymmetric cytokinesis in the mouse oocyte. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biology of reproduction

VOLUME: 76

Page Numbers: 949-57

Journal Abbreviation: Biol. Reprod.

ISSN: 0006-3363

DAY: 7

MONTH: 02

YEAR: 2007

Allocation of gamma-tubulin between oocyte cortex and meiotic spindle influences asymmetric cytokinesis in the mouse oocyte. Information

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LANGUAGE: eng

NlmUniqueID: 207224

Allocation of gamma-tubulin between oocyte cortex and meiotic spindle influences asymmetric cytokinesis in the mouse oocyte. Keywords Mesh Terms:

KEYWORDS: Tubulin

MESH TERMS: physiology

Chemical & Substance for Abstract: Allocation of gamma-tubulin between oocyte cortex and meiotic spindle influences asymmetric cytokinesis in the mouse oocyte. Information

Substance Name: Tubulin

Registry Number: 0

Grant and Affiliation Information for Allocation of gamma-tubulin between oocyte cortex and meiotic spindle influences asymmetric cytokinesis in the mouse oocyte.

AFFILIATION: Program in Cellular, Molecular and Developmental Biology, Tufts University School of Medicine, Boston, MA 02111, USA.

Country: United States

AGENCY: United States NICHD

GRANT: R01 HD 42076

ACRONYM: HD

MEDLINETA: Biol Reprod

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