Alkaliphilic Bacillus sp. strain KSM-LD1 contains a record number of subtilisin-like serine proteases genes.

Alkaliphilic Bacillus sp. strain KSM-LD1 contains a record number of subtilisin-like serine proteases genes. Research Abstract Details 

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Alkaliphilic Bacillus sp. strain KSM-LD1 contains a record number of subtilisin-like serine proteases genes. Abstract Text:

Yasushi Takimura,Kazuhiro Saito,Mitsuyoshi Okuda,Yasushi Kageyama,Katsuhisa Saeki,Katsuya Ozaki,Susumu Ito,Tohru Kobayashi,Yasushi Takimura,Kazuhiro Saito,Mitsuyoshi Okuda,Yasushi Kageyama,Katsuhisa Saeki,Katsuya Ozaki,Susumu Ito,Tohru Kobayashi,

The presence of 11 genes encoding subtilisin-like serine proteases was demonstrated by cloning from the genome of alkaliphilic Bacillus sp. strain KSM-LD1. This strain exoproduces the oxidatively stable alkaline protease LD-1 (Saeki et al. Curr Microbiol, 47:337-340, 2003). Among the 11 genes, six genes encoding alkaline proteases (SA, SB, SC, SD, SE, and LD-1) were expressed in Bacillus hosts. However, the other five genes for subtilisin-like proteases (SF, SG, SH, SI, and SJ) were expressed in neither Bacillus hosts nor Escherichia coli. The deduced amino acid sequences of SA, SB, SC, SF, SG, SH, SI, and SJ showed similarity to those of other subtilisin-like proteases from Bacillus strains with only 38 to 86% identity. The deduced amino acid sequence of SD was completely identical to that of an oxidatively stable alkaline protease from Bacillus sp. strain SD521, and that of SE was almost identical to that of a high-molecular mass subtilisin from Bacillus sp. strain D-6 with 99.7% identity. There are four to nine subtilisin-like serine protease genes in the reported genomes of Bacillus strains. At least 11 genes for the enzymes present in the genome of Bacillus sp. strain KSM-LD1, and this is the greatest number identified to date.

Alkaliphilic Bacillus sp. strain KSM-LD1 contains a record number of subtilisin-like serine proteases genes. Publishing Authors By Initials

Y Takimura,K Saito,M Okuda,Y Kageyama,K Saeki,K Ozaki,S Ito,T Kobayashi,Y Takimura,K Saito,M Okuda,Y Kageyama,K Saeki,K Ozaki,S Ito,T Kobayashi,

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Alkaliphilic Bacillus sp. strain KSM-LD1 contains a record number of subtilisin-like serine proteases genes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Applied microbiology and biotechnology

VOLUME: 76

Page Numbers: 395-405

Journal Abbreviation: Appl. Microbiol. Biotechnol.

ISSN: 0175-7598

DAY: 15

MONTH: 06

YEAR: 2007

Alkaliphilic Bacillus sp. strain KSM-LD1 contains a record number of subtilisin-like serine proteases genes. Information

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LANGUAGE: eng

NlmUniqueID: 8406612

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Grant and Affiliation Information for Alkaliphilic Bacillus sp. strain KSM-LD1 contains a record number of subtilisin-like serine proteases genes.

AFFILIATION: Tochigi Research Laboratories of Kao Corporation, Tochigi, Japan.

Country: Germany

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MEDLINETA: Appl Microbiol Biotechnol

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