Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions.

Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions. Research Abstract Details 

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Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions. Abstract Text:

Krishnamohan Atmakuri,Eric Cascales,Oliver T Burton,Lois M Banta,Peter J Christie,Krishnamohan Atmakuri,Eric Cascales,Oliver T Burton,Lois M Banta,Peter J Christie,

Agrobacterium tumefaciens translocates T-DNA through a polar VirB/D4 type IV secretion (T4S) system. VirC1, a factor required for efficient T-DNA transfer, bears a deviant Walker A and other sequence motifs characteristic of ParA and MinD ATPases. Here, we show that VirC1 promotes conjugative T-DNA transfer by stimulating generation of multiple copies per cell of the T-DNA substrate (T-complex) through pairwise interactions with the processing factors VirD2 relaxase, VirC2, and VirD1. VirC1 also associates with the polar membrane and recruits T-complexes to cell poles, the site of VirB/D4 T4S machine assembly. VirC1 Walker A mutations abrogate T-complex generation and polar recruitment, whereas the native protein recruits T-complexes to cell poles independently of other polar processing factors (VirC2, VirD1) or T4S components (VirD4 substrate receptor, VirB channel subunits). We propose that A. tumefaciens has appropriated a progenitor ParA/MinD-like ATPase to promote conjugative DNA transfer by: (i) nucleating relaxosome assembly at oriT-like T-DNA border sequences and (ii) spatially positioning the transfer intermediate at the cell pole to coordinate substrate-T4S channel docking.

Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions. Publishing Authors By Initials

K Atmakuri,E Cascales,OT Burton,LM Banta,PJ Christie,K Atmakuri,E Cascales,OT Burton,LM Banta,PJ Christie,

For similar biological factors: toxins, biological: virulence factors research abstracts see: biological factors: toxins, biological: virulence factors research

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Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The EMBO journal

VOLUME: 26

Page Numbers: 2540-51

Journal Abbreviation: EMBO J.

ISSN: 0261-4189

DAY: 16

MONTH: May

YEAR: 2007

Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8208664

Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions. Keywords Mesh Terms:

KEYWORDS: Virulence Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions. Information

Substance Name: Adenosine Triphosphatases

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Agrobacterium ParA/MinD-like VirC1 spatially coordinates early conjugative DNA transfer reactions.

AFFILIATION: Department of Microbiology and Molecular Genetics, University of Texas Medical School at Houston, Houston, TX 77030, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM48746

ACRONYM: GM

MEDLINETA: EMBO J

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