Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method.

Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Abstract Text:

Xiuren Zhang,Rossana Henriques,Shih-Shun Lin,Qi-Wen Niu,Nam-Hai Chua,Xiuren Zhang,Rossana Henriques,Shih-Shun Lin,Qi-Wen Niu,Nam-Hai Chua,

Collective efforts of several laboratories in the past two decades have resulted in the development of various methods for Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana. Among these, the floral dip method is the most facile protocol and widely used for producing transgenic Arabidopsis plants. In this method, transformation of female gametes is accomplished by simply dipping developing Arabidopsis inflorescences for a few seconds into a 5% sucrose solution containing 0.01-0.05% (vol/vol) Silwet L-77 and resuspended Agrobacterium cells carrying the genes to be transferred. Treated plants are allowed to set seed which are then plated on a selective medium to screen for transformants. A transformation frequency of at least 1% can be routinely obtained and a minimum of several hundred independent transgenic lines generated from just two pots of infiltrated plants (20-30 plants per pot) within 2-3 months. Here, we describe the protocol routinely used in our laboratory for the floral dip method for Arabidopsis transformation. Transgenic Arabidopsis plants can be obtained in approximately 3 months.

Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Publishing Authors By Initials

X Zhang,R Henriques,SS Lin,QW Niu,NH Chua,X Zhang,R Henriques,SS Lin,QW Niu,NH Chua,

For similar genetic processes: recombination, genetic: transformation, genetic research abstracts see: genetic processes: recombination, genetic: transformation, genetic research

PUBMED ID PMID:

MEDLINE DATE:

Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nature protocols

VOLUME: 1

Page Numbers: 641-6

Journal Abbreviation:

ISSN: 1750-2799

DAY: 29

MONTH: 06

YEAR: 2006

Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101284307

Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Keywords Mesh Terms:

KEYWORDS: Transformation, Genetic

MESH TERMS: genetics

Chemical & Substance for Abstract: Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method.

AFFILIATION: Laboratory of Plant Molecular Biology, Rockefeller University, 1230 York Avenue, New York, New York 10021, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM 44640

ACRONYM: GM

MEDLINETA: Nat Protoc

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method Related Publications

• ABA induction of miR159 controls transcript levels of two MYB factors during Arabidopsis seed germination.
• Acute urinary retention associated with selective serotonin reuptake inhibitors and ziprasidone.
• Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method.
• An outbreak of echovirus 11 amongst neonates in a confinement home in Penang, Malaysia.
• Angiotensin converting enzyme activity in strains of normo- and hypertensive rats.
• Body mass index as a screening test for metabolic syndrome in schizophrenia and schizoaffective disorders.
• Chikungunya virus of Central/East African genotype detected in Malaysia.
• Complicated infantile meningitis: evaluation by real-time sonography.
• Control of quorum sensing by a Burkholderia pseudomallei multidrug efflux pump.
• Cribside neurosonography: real-time sonography for intracranial investigation of the neonate.
• Dual-target interference for the 'automatic pilot' in the dorsal stream.
• Factors influencing radial artery size.
• Human interlimb reflexes evoked by electrical stimulation of cutaneous nerves innervating the hand and foot.
• Independent and interdependent functions of LAF1 and HFR1 in phytochrome A signaling.
• Light signal transduction in plants.
• Modulation of human cutaneous reflexes during rhythmic cyclical arm movement.
• Morphology and autowave metric on CNN applied to bubble-debris classification.
• Necrostatin-1 protects against glutamate-induced glutathione depletion and caspase-independent cell death in HT-22 cells.
• No automatic pilot for visually guided aiming based on colour.
• Obstructed breathing during sleep and obstructive sleep apnoea syndrome--assessment and treatment.
• Overexpression of IAP-2 attenuates apoptosis and protects against myocardial ischemia/reperfusion injury in transgenic mice.
• Prevalence of Sexual Problems and Its Association with Social, Psychological and Physical Factors among Men in a Malaysian Population: A Cross-Sectional Study.
• Relative contributions of visual and vestibular information on the trajectory of human gait.
• Resistin, an adipocytokine, offers protection against acute myocardial infarction.
• Spatial constraints in bimanual coordination: influences of effector orientation.
• Sympathetic activity and essential hypertension.
• Targeted degradation of PSEUDO-RESPONSE REGULATOR5 by an SCFZTL complex regulates clock function and photomorphogenesis in Arabidopsis thaliana.
• The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses.
• The experience of occluding secundum atrial septal defect.
• [Bilateral cochlear implantation in a post-lingually deafened Mandarin-speaking patient]