Agonist- and T(H)2 cytokine-induced up-regulation of cysteinyl leukotriene receptor messenger RNA in human monocytes.

Agonist- and T(H)2 cytokine-induced up-regulation of cysteinyl leukotriene receptor messenger RNA in human monocytes. Research Abstract Details 

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Agonist- and T(H)2 cytokine-induced up-regulation of cysteinyl leukotriene receptor messenger RNA in human monocytes. Abstract Text:

Hideaki Shirasaki,Nobuhiko Seki,Manabu Fujita,Megumi Kikuchi,Etsuko Kanaizumi,Kazumasa Watanabe,Tetsuo Himi,Hideaki Shirasaki,Nobuhiko Seki,Manabu Fujita,Megumi Kikuchi,Etsuko Kanaizumi,Kazumasa Watanabe,Tetsuo Himi,

BACKGROUND: The cysteinyl leukotrienes (CysLTs) are lipid mediators that have been implicated in the pathogenesis of allergic diseases, and their actions are mediated via specific receptors named CysLT1 receptor (CysLT1R) and CysLT2 receptor (CysLT2R). Little information is known about the role of T(H)2 cytokines in the regulation of both CysLT1R and CysLT2R expression. OBJECTIVE: To investigate the possible modulation of both CysLT1R and CysLT2R messenger RNA (mRNA) expression, we have developed a real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay based on the TaqMan fluorescence method to quantify CysLT1R and CysLT2R mRNA in human monocytes. METHODS: Human monocytes were stimulated with leukotriene D4 or interleukin (IL) 4 or IL-13, and the levels of CysLT1R and CysLT2R mRNA were measured by the quantitative RT-PCR. RESULTS: CysLT1R and CysLT2R mRNA was increased after stimulation with leukotriene D4. CysLT1R mRNA was augmented 150-fold after treatment with IL-4; however, no significant increase was observed in CysLT2R mRNA level. IL-13 could induce a biphasic augmentation of CysLT1R mRNA level. In contrast to IL-4, IL-13 enhanced CysLT2R mRNA level, with a maximal effect at 2 hours of incubation. CONCLUSIONS: CysLT1R and CysLT2R expression can be regulated by CysLT itself and T(H)2 cytokines at the transcriptional level.

Agonist- and T(H)2 cytokine-induced up-regulation of cysteinyl leukotriene receptor messenger RNA in human monocytes. Publishing Authors By Initials

H Shirasaki,N Seki,M Fujita,M Kikuchi,E Kanaizumi,K Watanabe,T Himi,H Shirasaki,N Seki,M Fujita,M Kikuchi,E Kanaizumi,K Watanabe,T Himi,

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Agonist- and T(H)2 cytokine-induced up-regulation of cysteinyl leukotriene receptor messenger RNA in human monocytes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Annals of allergy, asthma & immunology : official

VOLUME: 99

Page Numbers: 340-7

Journal Abbreviation: Ann. Allergy Asthma Immunol.

ISSN: 1081-1206

DAY: 18

MONTH: Oct

YEAR: 2007

Agonist- and T(H)2 cytokine-induced up-regulation of cysteinyl leukotriene receptor messenger RNA in human monocytes. Information

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LANGUAGE: eng

NlmUniqueID: 9503580

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AFFILIATION: Department of Otolaryngology, Sapporo Medical University, Chuo-ku, Sapporo, Japan. shira@sapmed.ac.jp

Country: United States

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MEDLINETA: Ann Allergy Asthma Immunol

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