Aging impact on biochemical activities and gene expression of Drosophila melanogaster mitochondria.

Aging impact on biochemical activities and gene expression of Drosophila melanogaster mitochondria. Research Abstract Details 

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Aging impact on biochemical activities and gene expression of Drosophila melanogaster mitochondria. Abstract Text:

Pascal Dubessay,Isabelle Garreau-Balandier,Anne-Sophie Jarrousse,Annie Fleuriet,Benoit Sion,Roger Debise,Serge Alziari,

The consequences of aging are characterized by a decline in the main cellular functions, including those of the mitochondria. Although these consequences have been much studied, efforts have often focused solely on a few parameters used to assess the "state" of mitochondrial function during aging. We performed comparative measurements of several parameters in young (a few days) and old (8 and 12 weeks) adult male Drosophila melanogaster: respiratory complex activities, mitochondrial respiration, ATP synthesis, lipid composition of the inner membrane, concentrations of respiratory complex subunits, expression of genes (nuclear and mitochondrial) coding for mitochondrial proteins. Our results show that, in the mitochondria of "old" flies, the activities of three respiratory complexes (I, III, IV) are greatly diminished, ATP synthesis is decreased, and the lipid composition of the inner membrane (fatty acids, cardiolipin) is modified. However, the respiration rate and subunit concentrations measured by Western blot are unaffected. Although cellular mitochondrial DNA (mtDNA) content remains constant, there is a decrease in concentrations of nuclear and mitochondrial transcripts apparently coordinated. The expression of nuclear genes encoding the transcription factors TFAM, TFB1, TFB2, and DmTTF, which are essential for the maintenance and expression of mtDNA are also decreased. The decrease in nuclear and mitochondrial transcript concentrations may be one of the principal effects of aging on mitochondria, and could explain observed decreases in mitochondrial efficiency.

Aging impact on biochemical activities and gene expression of Drosophila melanogaster mitochondria. Publishing Authors By Initials

P Dubessay,I Garreau-Balandier,AS Jarrousse,A Fleuriet,B Sion,R Debise,S Alziari,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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Aging impact on biochemical activities and gene expression of Drosophila melanogaster mitochondria. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biochimie

VOLUME: 89

Page Numbers: 988-1001

Journal Abbreviation: Biochimie

ISSN: 0300-9084

DAY: 4

MONTH: 04

YEAR: 2007

Aging impact on biochemical activities and gene expression of Drosophila melanogaster mitochondria. Information

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LANGUAGE: eng

NlmUniqueID: 1264604

Aging impact on biochemical activities and gene expression of Drosophila melanogaster mitochondria. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

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Substance Name: RNA

Registry Number: 63231-63-0

Grant and Affiliation Information for Aging impact on biochemical activities and gene expression of Drosophila melanogaster mitochondria.

AFFILIATION: Equipe Génome Mitochondrial, UMR CNRS 6547, Université Blaise Pascal-Clermont II, 63177 Aubière, France.

Country: France

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MEDLINETA: Biochimie

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