Aging does not affect spermatogenic recovery after experimentally induced injury in mice.

Aging does not affect spermatogenic recovery after experimentally induced injury in mice. Research Abstract Details 

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Aging does not affect spermatogenic recovery after experimentally induced injury in mice. Abstract Text:

Jens Ehmcke,Bhavika Joshi,Scott D Hergenrother,Stefan Schlatt,

Testes in aging mammals undergo a variety of age-related changes, such as reduction of size, lower sperm output, an increase in abnormal forms of sperm, and endocrine malfunctions. It has been suggested that the spermatogenic defects are due to loss and dysfunction of spermatogonial stem cells as well as deterioration of the tubule microenvironment. In the present study, we explore the depletion and recovery of spermatogenesis in young (3 month) and old (12 month) mice exposed to cooling, X-irradiation (5 Gy) or cytotoxic treatment using Busulfan (40 mg/kg). We aim to determine a potential age-related change of vulnerability to gonadotoxic treatments by describing the intensity of spermatogenic depletion and the degree of spermatogenic recolonization with qualitative and quantitative parameters on organ weights and histological parameters at two time points (2 weeks, depletion; 6 weeks, recovery). Our data reveal specific acute effects of cooling on multinucleation of germ cells but no other severe injury. Irradiation and Busulfan-treatment exerted the expected depletional wave of germ cells leading to severe testicular injury and spermatogenic failure. The recovery of spermatogenesis occurred in both treatment groups and both age groups to a similar extent. We therefore noted no prominent age-related differences in spermatogenic depletion and recovery in any treatment group. We conclude that in both age groups, the remaining spermatogonial stem cells are capable to induce spermatogenic recovery and the aging tubule microenvironment at 1 year has not become more vulnerable to irradiation, Busulfan-treatment or testicular cooling.

Aging does not affect spermatogenic recovery after experimentally induced injury in mice. Publishing Authors By Initials

J Ehmcke,B Joshi,SD Hergenrother,S Schlatt,

For similar natural sciences: physics: radiation: radiation, ionizing: x-rays research abstracts see: natural sciences: physics: radiation: radiation, ionizing: x-rays research

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Aging does not affect spermatogenic recovery after experimentally induced injury in mice. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Reproduction (Cambridge, England)

VOLUME: 133

Page Numbers: 75-83

Journal Abbreviation: Reproduction

ISSN: 1470-1626

DAY: 3

MONTH: Jan

YEAR: 2007

Aging does not affect spermatogenic recovery after experimentally induced injury in mice. Information

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LANGUAGE: eng

NlmUniqueID: 100966036

Aging does not affect spermatogenic recovery after experimentally induced injury in mice. Keywords Mesh Terms:

KEYWORDS: X-Rays

MESH TERMS: adverse effects

Chemical & Substance for Abstract: Aging does not affect spermatogenic recovery after experimentally induced injury in mice. Information

Substance Name: Busulfan

Registry Number: 55-98-1

Grant and Affiliation Information for Aging does not affect spermatogenic recovery after experimentally induced injury in mice.

AFFILIATION: Department of Cell Biology, University of Pittsburgh School of Medicine, W952 Biomedical Science Towers, 3500 Terrace Street, Pittsburgh 15261, Pennsylvania, USA.

Country: England

AGENCY: United States NIA

GRANT: 5R21 AG024914-02

ACRONYM: AG

MEDLINETA: Reproduction

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