Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice.

Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice. Research Abstract Details 

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Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice. Abstract Text:

Heping Xu,Mei Chen,Ayyakkannu Manivannan,Noemi Lois,John V Forrester,

Fundus autofluorescence (AF) imaging by confocal scanning laser ophthalmoscopy has been widely used by ophthalmologists in the diagnosis/monitoring of various retinal disorders. It is believed that fundus AF is derived from lipofuscin in retinal pigment epithelial (RPE) cells; however, direct clinicopathological correlation has not been possible in humans. We examined fundus AF by confocal scanning laser ophthalmoscopy and confocal microscopy in normal C57BL/6 mice of different ages. Increasingly strong AF signals were observed with age in the neuroretina and subretinal/RPE layer by confocal scanning laser ophthalmoscopy. Unlike fundus AF detected in normal human subjects, mouse fundus AF appeared as discrete foci distributed throughout the retina. Most of the AF signals in the neuroretina were distributed around retinal vessels. Confocal microscopy of retinal and choroid/RPE flat mounts demonstrated that most of the AF signals were derived from Iba-1+ perivascular and subretinal microglia. An age-dependent accumulation of Iba-1+ microglia at the subretinal space was observed. Lipofuscin granules were detected in large numbers in subretinal microglia by electron microscopy. The number of AF+ microglia and the amount of AF granules/cell increased with age. AF granules/lipofuscin were also observed in RPE cells in mice older than 12 months, but the number of AF+ RPE cells was very low (1.48 mm(-2) and 5.02 mm(-2) for 12 and 24 months, respectively) compared to the number of AF+ microglial cells (20.63 mm(-2) and 76.36 mm(-2) for 6 and 24 months, respectively). The fluorescence emission fingerprints of AF granules in subretinal microglia were the same as those in RPE cells. Our observation suggests that perivascular and subretinal microglia are the main cells producing lipofuscin in normal aged mouse retina and are responsible for in vivo fundus AF. Microglia may play an important role in retinal aging and age-related retinal diseases.

Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice. Publishing Authors By Initials

H Xu,M Chen,A Manivannan,N Lois,JV Forrester,

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Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Aging cell

VOLUME: 7

Page Numbers: 58-68

Journal Abbreviation: Aging Cell

ISSN: 1474-9726

DAY: 26

MONTH: 11

YEAR: 2007

Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Age-dependent accumulation of lipofuscin in perivascular and subretinal microglia in experimental mice.

AFFILIATION: Department of Ophthalmology, Institute of Medical Sciences, School of Medicine, University of Aberdeen, Scotland, UK. h.xu@abdn.ac.uk

Country: England

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MEDLINETA: Aging Cell

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