Affinity separation of an acid phosphatase from rat tissues and Gaucher spleen with immobilized Cibacron Blue.

Affinity separation of an acid phosphatase from rat tissues and Gaucher spleen with immobilized Cibacron Blue. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Affinity separation of an acid phosphatase from rat tissues and Gaucher spleen with immobilized Cibacron Blue. Abstract Text:

A Hara,H Sawada,T Nakayama,Y Matsumoto,

An acid phosphatase species which was activated by Fe2+ was determined to be partially soluble but mainly particulate in rat spleen. The particulate enzyme could be extracted into 1 M KCl. This enzyme bound to Cibacron Blue-immobilized Sepharose (Blue-Sepharose) and was desorbed by 2 M KCl with a good yield, while the other acid phosphatases in rat spleen did not adsorb on Blue-Sepharose. The enzymes eluted on Blue-Sepharose chromatography of both the soluble and particulate fractions were electrophoretically identical. The enzyme hydrolyzed aryl monophosphates, phosphoproteins, and nucleoside di- and triphosphates. The activity for the three kinds of substrate was similarly activated by Fe2+, ascorbic acid and cysteine, and inhibited by molybdate, Cu2+ and F-. Cibacron Blue inhibited the enzyme competitively with respect to a substrate, p-nitrophenyl phosphate, but kinetic analysis suggested that more than one dye molecule binds to the enzyme. The Blue-Sepharose technique could be applied not only to quantitative separation of acid phosphatases similar to the spleen enzyme from bone and epidermis of rat, but also to that of a tartrate-resistant acid phosphatase from human spleen with Gaucher's disease.

Affinity separation of an acid phosphatase from rat tissues and Gaucher spleen with immobilized Cibacron Blue. Publishing Authors By Initials

A Hara,H Sawada,T Nakayama,Y Matsumoto,

For similar tissues: lymphoid tissue: spleen research abstracts see: tissues: lymphoid tissue: spleen research

PUBMED ID PMID:

MEDLINE DATE:

Affinity separation of an acid phosphatase from rat tissues and Gaucher spleen with immobilized Cibacron Blue. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 94

Page Numbers: 721-7

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1983

Affinity separation of an acid phosphatase from rat tissues and Gaucher spleen with immobilized Cibacron Blue. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Affinity separation of an acid phosphatase from rat tissues and Gaucher spleen with immobilized Cibacron Blue. Keywords Mesh Terms:

KEYWORDS: Spleen

MESH TERMS: enzymology

Chemical & Substance for Abstract: Affinity separation of an acid phosphatase from rat tissues and Gaucher spleen with immobilized Cibacron Blue. Information

Substance Name: Acid Phosphatase

Registry Number: EC 3.1.3.2

Grant and Affiliation Information for Affinity separation of an acid phosphatase from rat tissues and Gaucher spleen with immobilized Cibacron Blue.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Affinity separation of an acid phosphatase from rat tissues and Gaucher spleen with immobilized Cibacron Blue Related Publications

• A Third Locus for Eosinophilia on Chromosome 1 of the MES Rats.
• Anticariogenic effect of fluoride-releasing elastomers in orthodontic patients.
• Complement 3 is involved in changing the phenotype of human glomerular mesangial cells.
• Crystal structure, mesomorphic and emission properties of liquid crystals having a 7-(diethylamino)-3-phenylcoumarin core.
• Dental-skeletal dimensions in growing individuals with variations in the lower facial height.
• Durability of endourologic skills: two-year follow-up study.
• Effects of IL-6 and soluble IL-6 receptor on the expression of cartilage matrix proteins in human chondrocytes.
• Epistasis between hyperglycemic QTLs revealed in a double congenic of the OLETF rat.
• Estrogen promotes early osteoblast differentiation and inhibits adipocyte differentiation in mouse bone marrow stromal cell lines that express estrogen receptor (ER) alpha or beta.
• Fundamental aspects of hypogonadism in the aging male.
• Glossopharyngeal neuralgia due to an epidermoid tumour in the cerebellopontine angle.
• Mammographic x-ray spectra measured by Compton spectroscopy using a high resolution Schottky CdTe detector.
• Microbubble-induced increase in ablation of liver tumors by high-intensity focused ultrasound.
• N-[2-(m-methoxyphenyl)ethyl]-N-ethyl-2-(1-pyrrolidinyl)ethylamine (UMB 116) is a novel antagonist for cocaine-induced effects.
• Novel eye-specific calmodulin methylation characterized by protein mapping in Drosophila melanogaster.
• Novel sigma (sigma) receptor agonists produce antidepressant-like effects in mice.
• Optic nerve decompression for orbitofrontal fibrous dysplasia.
• Perforation of Meckel's diverticulum manifesting as aseptic peritonitis in a neonate: report of a case.
• Proteomics study of neuropathic and nonneuropathic dorsal root ganglia: altered protein regulation following segmental spinal nerve ligation injury.
• Radiofrequency ablation and cryoablation of renal tumours.
• Rapid detection of human herpesvirus 8 DNA using loop-mediated isothermal amplification.
• Segmental alveolar distraction for the correction of unilateral open-bite caused by multiple ankylosed teeth: A case report.
• Sex pheromone production in the silkworm, Bombyx mori, is mediated by store-operated Ca2+ channels.
• Somatostatin inhibits the excitability of rat small-diameter trigeminal ganglion neurons that innervate nasal mucosa and project to the upper cervical dorsal horn via activation of somatostatin 2a receptor.
• Synthesis of D-labeled naphthyliodoacetamide and application to quantitative peptide analysis by isotope differential mass spectrometry.
• The Bombyx mori sex pheromone biosynthetic pathway is not mediated by cAMP.
• The Dugesia ryukyuensis database as a molecular resource for studying switching of the reproductive system.
• Therapy with bortezomib plus dexamethasone induces osteoblast activation in responsive patients with multiple myeloma.
• [Evaluation of the accuracy of line spread function (LSF) and point spread function (PSF) measured in the computed tomography.]
• [Phosphatonin]