Affinity selection of DNA-binding proteins from yeast genomic DNA libraries by improved lambda phage display vector.

Affinity selection of DNA-binding proteins from yeast genomic DNA libraries by improved lambda phage display vector. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Affinity selection of DNA-binding proteins from yeast genomic DNA libraries by improved lambda phage display vector. Abstract Text:

Hiroko Hagiwara,Sumiko Kunihiro,Keiichi Nakajima,Motoaki Sano,Haruhiko Masaki,Midori Yamamoto,Jeong Won Pak,Yan Zhang,Kumiko Takase,Ichiro Kuwabara,Ichiro N Maruyama,Masayuki Machida,

Phage display is a useful means of identifying and selecting proteins of interest that bind specific targets. In order to examine the potential of phage display for the genome-wide screening of DNA-binding proteins, we constructed yeast genomic libraries using lambda foo-based vectors devised in this work. After affinity selection using GAL4 UAS(G) as a probe, phages expressing GAL4 were enriched approximately 5 x 10(5)-fold from the library. Approximately 90% of polypeptides encoded in correct translation reading frames by the selected phages were known or putative polynucleotide-binding proteins. This result clearly indicates that the modified lambda phage display vector in combination with our enrichment technique has great potential for the enrichment of DNA-binding proteins in a sequence-specific manner.

Affinity selection of DNA-binding proteins from yeast genomic DNA libraries by improved lambda phage display vector. Publishing Authors By Initials

H Hagiwara,S Kunihiro,K Nakajima,M Sano,H Masaki,M Yamamoto,JW Pak,Y Zhang,K Takase,I Kuwabara,IN Maruyama,M Machida,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

PUBMED ID PMID:

MEDLINE DATE:

Affinity selection of DNA-binding proteins from yeast genomic DNA libraries by improved lambda phage display vector. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 132

Page Numbers: 975-82

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 2002

Affinity selection of DNA-binding proteins from yeast genomic DNA libraries by improved lambda phage display vector. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Affinity selection of DNA-binding proteins from yeast genomic DNA libraries by improved lambda phage display vector. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Affinity selection of DNA-binding proteins from yeast genomic DNA libraries by improved lambda phage display vector. Information

Substance Name: Transcription Factors

Registry Number: 0

Grant and Affiliation Information for Affinity selection of DNA-binding proteins from yeast genomic DNA libraries by improved lambda phage display vector.

AFFILIATION: Research Center for Glycoscience, Advanced Institute of Industrial Science and Technology, Central Higashi, Tsukuba, Ibaraki 305-8566, Japan.

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Affinity selection of DNA-binding proteins from yeast genomic DNA libraries by improved lambda phage display vector Related Publications

• A MAP kinase cascade that controls plant cytokinesis.
• A novel vector for positive selection of inserts harboring an open reading frame by translational coupling.
• Affinity selection of DNA-binding proteins displayed on bacteriophage lambda.
• Affinity selection of DNA-binding proteins from yeast genomic DNA libraries by improved lambda phage display vector.
• An oncoprotein from the plant pathogen agrobacterium has histone chaperone like activity.
• Application of chitin and chitosan derivatives in the pharmaceutical field.
• Deletion analysis of the catalase-encoding gene (catB) promoter from Aspergillus oryzae.
• Development of an automated SNP analysis method using a paramagnetic beads handling robot.
• Effect of iontophoresis and switching iontophoresis on skin accumulation of ketoprofen.
• Expression and Characterization of Recombinant C-Terminal Biotinylated Extracellular Domain of Human Receptor for Advanced Glycation End Products (hsRAGE) in Escherichia coli.
• Field-Angle-Dependent Specific Heat Measurements and Gap Determination of a Heavy Fermion Superconductor URu2Si2.
• Formulation and evaluation of lactoferrin bioadhesive tablets.
• Gastrointestinal distribution and absorption behavior of Eudragit-coated chitosan-prednisolone conjugate microspheres in rats with TNBS-induced colitis.
• HISTOCHEMISTRY AND CYTOCHEMISTRY OF HUMAN SKIN. XXVI. ALKALINE PHOSPHATASE ACTIVITY IN THE SENSORY NERVE ENDINGS OF THE CLITORIS.
• HISTOLOGY AND CYTOCHEMISTRY OF HUMAN SKIN. XXV. COMMON ABNORMALITIES IN THE ECCRINE SWEAT GLANDS OF MAN.
• In vitro Evaluation of Cinnarizine as a Competing Agent to beta-Cyclodextrin Inclusion Complexes: Effect of Cinnarizine on the Membrane Permeation Rate of Progesterone from Its beta-Cyclodextrin Inclusion Complex.
• In vivo evaluation of Kumazasa extract and chitosan films containing the extract against deep skin ulcer model in rats.
• Novel preparation of enteric-coated chitosan-prednisolone conjugate microspheres and in vitro evaluation of their potential as a colonic delivery system.
• Particle Characteristics and Biodistribution of Camptothecin-Loaded PLA/(PEG-PPG-PEG) Nanoparticles.
• Preparation of double liposomes and their efficiency as an oral vaccine carrier.
• Spinor dipolar bose-einstein condensates: classical spin approach.
• Splenic abscess in an infant caused by Streptococcus intermedius.
• Successful prosthetic fitting of elderly trans-femoral amputees with Intelligent Prosthesis (IP): a clinical pilot study.
• Synthesis of indolyl aryl 1,2-diketones.
• Transcriptional regulation of genes on the non-syntenic blocks of Aspergillus oryzae and its functional relationship to solid-state cultivation.
• Vitrification of mouse oocytes and embryos at various stages of development in an ethylene glycol-based solution by a simple method.
• [An surgical case of right pulmonary coccidioidomycosis--with subcutaneous coccidiomycosis in the left wrist]
• [Application and innovation of aortocoronary bypass--evaluation of application based on experimental studies]
• [Bone disease with Pain. Pyogenic vertebral osteomyelitis.]
• [Method of SNR determination using clinical images]