Aerosol delivery of Akt controls protein translation in the lungs of dual luciferase reporter mice.

Aerosol delivery of Akt controls protein translation in the lungs of dual luciferase reporter mice. Research Abstract Details 

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Aerosol delivery of Akt controls protein translation in the lungs of dual luciferase reporter mice. Abstract Text:

A M Tehrani,S-K Hwang,T-H Kim,C-S Cho,J Hua,W-S Nah,J-T Kwon,J-S Kim,S-H Chang,K-N Yu,S-J Park,D R Bhandari,K-H Lee,G-H An,G R Beck,M-H Cho,

Lung cancer has emerged as a leading cause of cancer death in the world; however, most of the current conventional therapies are not sufficiently effective in altering the progression of disease. Therefore, development of novel treatment approaches is needed. Although several genes and methods have been used for cancer gene therapy, a number of problems such as specificity, efficacy and toxicity reduce their application. This has led to re-emergence of aerosol gene delivery as a noninvasive method for lung cancer treatment. In this study, nano-sized glucosylated polyethyleneimine (GPEI) was used as a gene delivery carrier to investigate the effects of Akt wild type (WT) and kinase deficient (KD) on Akt-related signaling pathways and protein translation in the lungs of CMV- LucR-cMyc-IRES-LucF dual reporter mice. These mice are a powerful tool for the discrimination between cap-dependent/-independent protein translation. Aerosols containing self-assembled nano-sized GPEI/Akt WT or GPEI/Akt KD were delivered into the lungs of reporter mice through nose-only-inhalation-chamber with the aid of nebulizer. Aerosol delivery of Akt WT caused the increase of protein expression levels of Akt-related signals, whereas aerosol delivery of Akt KD did not. Furthermore, dual luciferase activity assay showed that aerosol delivery of Akt WT enhanced cap-dependent protein translation, whereas a reduction in cap-dependent protein translation by Akt KD was observed. Our results clearly showed that targeting Akt may be a good strategy for prevention as well as treatment of lung cancer. These studies suggest that our aerosol delivery is compatible for in vivo gene delivery which could be used as a noninvasive gene therapy in the future.

Aerosol delivery of Akt controls protein translation in the lungs of dual luciferase reporter mice. Publishing Authors By Initials

AM Tehrani,SK Hwang,TH Kim,CS Cho,J Hua,WS Nah,JT Kwon,JS Kim,SH Chang,KN Yu,SJ Park,DR Bhandari,KH Lee,GH An,GR Beck,MH Cho,

For similar enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: proto-oncogene proteins c-akt research abstracts see: enzymes and coenzymes: enzymes: transferases: phosphotransferases: phosphotransferases (alcohol group acceptor): protein kinases: protein-serine-threonine kinases: proto-oncogene proteins c-akt research

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Aerosol delivery of Akt controls protein translation in the lungs of dual luciferase reporter mice. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Gene therapy

VOLUME: 14

Page Numbers: 451-8

Journal Abbreviation: Gene Ther.

ISSN: 0969-7128

DAY: 19

MONTH: 10

YEAR: 2006

Aerosol delivery of Akt controls protein translation in the lungs of dual luciferase reporter mice. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9421525

Aerosol delivery of Akt controls protein translation in the lungs of dual luciferase reporter mice. Keywords Mesh Terms:

KEYWORDS: Proto-Oncogene Proteins c-akt

MESH TERMS: genetics

Chemical & Substance for Abstract: Aerosol delivery of Akt controls protein translation in the lungs of dual luciferase reporter mice. Information

Substance Name: Proto-Oncogene Proteins c-akt

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Aerosol delivery of Akt controls protein translation in the lungs of dual luciferase reporter mice.

AFFILIATION: Laboratory of Toxicology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul, Korea.

Country: England

AGENCY: United States NCI

GRANT: CA84573

ACRONYM: CA

MEDLINETA: Gene Ther

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