Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening.

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening. Research Abstract Details 

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Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening. Abstract Text:

Ekaterina I Galanzha,Valery V Tuchin,Vladimir P Zharov,

Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo. Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including in vivo label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cellos functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo- and radio-sensitivity tests, and drug screening, are also discussed.

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening. Publishing Authors By Initials

EI Galanzha,VV Tuchin,VP Zharov,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats research

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Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening. Journal Published:

PUBLICATION TYPE: Review

Journal: World journal of gastroenterology : WJG

VOLUME: 13

Page Numbers: 192-218

Journal Abbreviation: World J. Gastroenterol.

ISSN: 1007-9327

DAY: 14

MONTH: Jan

YEAR: 2007

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening. Information

Number of References: 201

LANGUAGE: eng

NlmUniqueID: 100883448

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening. Keywords Mesh Terms:

KEYWORDS: Rats

MESH TERMS: methods

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Grant and Affiliation Information for Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening.

AFFILIATION: Philips Classic Laser Laboratories, University of Arkansas for Medical Sciences, 4301 W. Markham St., Little Rock, AR 72205-7199, United States. egalanzha@uams.edu

Country: China

AGENCY: United States NIBIB

GRANT: EB005123

ACRONYM: EB

MEDLINETA: World J Gastroenterol

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