Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution.

Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution. Research Abstract Details 

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Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution. Abstract Text:

Vicki L Sutherland,Jerilyn A Timlin,Linda T Nieman,John F Guzowski,Monica K Chawla,Paul F Worley,Badri Roysam,Bruce L McNaughton,Michael B Sinclair,Carol A Barnes,

Simultaneous imaging of multiple cellular components is of tremendous importance in the study of complex biological systems, but the inability to use probes with similar emission spectra and the time consuming nature of collecting images on a confocal microscope are prohibitive. Hyperspectral imaging technology, originally developed for remote sensing applications, has been adapted to measure multiple genes in complex biological tissues. A spectral imaging microscope was used to acquire overlapping fluorescence emissions from specific mRNAs in brain tissue by scanning the samples using a single fluorescence excitation wavelength. The underlying component spectra obtained from the samples are then separated into their respective spectral signatures using multivariate analyses, enabling the simultaneous quantitative measurement of multiple genes either at regional or cellular levels.

Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution. Publishing Authors By Initials

VL Sutherland,JA Timlin,LT Nieman,JF Guzowski,MK Chawla,PF Worley,B Roysam,BL McNaughton,MB Sinclair,CA Barnes,

For similar animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research abstracts see: animals: chordata: vertebrates: mammals: rodentia: muridae: murinae: rats: rats, sprague-dawley research

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Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of neuroscience methods

VOLUME: 160

Page Numbers: 144-8

Journal Abbreviation: J. Neurosci. Methods

ISSN: 0165-0270

DAY: 17

MONTH: 10

YEAR: 2006

Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution. Information

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LANGUAGE: eng

NlmUniqueID: 7905558

Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution. Keywords Mesh Terms:

KEYWORDS: Rats, Sprague-Dawley

MESH TERMS: metabolism

Chemical & Substance for Abstract: Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution. Information

Substance Name: RNA, Messenger

Registry Number: 0

Grant and Affiliation Information for Advanced imaging of multiple mRNAs in brain tissue using a custom hyperspectral imager and multivariate curve resolution.

AFFILIATION: Arizona Research Laboratories, Division of Neural Systems Memory & Aging, University of Arizona, Tucson, AZ, United States.

Country: Netherlands

AGENCY: United States NIA

GRANT: R01 AG023309-03

ACRONYM: AG

MEDLINETA: J Neurosci Methods

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