Advanced glycation endproducts influence the mRNA expression of RAGE, RANKL and various osteoblastic genes in human osteoblasts.

Advanced glycation endproducts influence the mRNA expression of RAGE, RANKL and various osteoblastic genes in human osteoblasts. Research Abstract Details 

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Advanced glycation endproducts influence the mRNA expression of RAGE, RANKL and various osteoblastic genes in human osteoblasts. Abstract Text:

Sybille Franke,Heide Siggelkow,Gunter Wolf,Gert Hein,Sybille Franke,Heide Siggelkow,Gunter Wolf,Gert Hein,Sybille Franke,Heide Siggelkow,Gunter Wolf,Gert Hein,Sybille Franke,Heide Siggelkow,Gunter Wolf,Gert Hein,

Glycation reactions resulting in the generation and accumulation of advanced glycation endproducts (AGEs) are potential mechanisms by which bone protein may be altered in vivo. AGEs accumulate in the bone increasingly with age come into close contact with osteoblasts or osteoclasts. The direct effect of AGEs on bone cells has not been thoroughly investigated. This study aimed to examine whether glycated bovine serum albumin (AGE - BSA) as an AGE modulate the mRNA expression of various genes in primary human osteoblast cultures. The following parameters were included: RAGE (receptor for AGEs), alkaline phosphatase, osteocalcin, osterix and RANKL (receptor activator of nuclear factor-kappaB ligand). Primary human osteoblast cultures were obtained from bone specimens of six patients with osteoarthrosis. Human osteoblasts were treated in AGE - BSA or control-BSA (non-glycated BSA) containing medium (5 mg/ml each) over a time course of seven days. After RT-PCR the mRNA expression was measured by real-time PCR. Related to control - BSA exposure, the mRNA expression of RAGE, RANKL and osterix increased during AGE - BSA treament. For alkaline phosphatase and osteocalcin a tendency of down-regulation was found. In summary, the study presents evidence that advanced glycation end products accumulated in bone alter osteoblasts by activation the AGE - RAGE pathway (RAGE mRNA up-regulation), inducing enhanced osteoclastogenesis (RANKL mRNA up-regulation) and impaired matrix mineralization (down-regulation of alkaline phosphatase and osteocalcin mRNA). Thus, AGEs may play a functional role in the development of bone diseases (e.g. osteoporosis).

Advanced glycation endproducts influence the mRNA expression of RAGE, RANKL and various osteoblastic genes in human osteoblasts. Publishing Authors By Initials

S Franke,H Siggelkow,G Wolf,G Hein,S Franke,H Siggelkow,G Wolf,G Hein,S Franke,H Siggelkow,G Wolf,G Hein,S Franke,H Siggelkow,G Wolf,G Hein,

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Advanced glycation endproducts influence the mRNA expression of RAGE, RANKL and various osteoblastic genes in human osteoblasts. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Archives of physiology and biochemistry

VOLUME: 113

Page Numbers: 154-61

Journal Abbreviation: Arch. Physiol. Biochem.

ISSN: 1381-3455

DAY: 8

MONTH: Jun

YEAR: 2007

Advanced glycation endproducts influence the mRNA expression of RAGE, RANKL and various osteoblastic genes in human osteoblasts. Information

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LANGUAGE: eng

NlmUniqueID: 9510153

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Grant and Affiliation Information for Advanced glycation endproducts influence the mRNA expression of RAGE, RANKL and various osteoblastic genes in human osteoblasts.

AFFILIATION: Clinic of Internal Medicine III, Friedrich-Schiller-University Jena, Germany.

Country: England

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MEDLINETA: Arch Physiol Biochem

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