Advanced environmental surveillance and molecular analyses indicate separate importations rather than endemic circulation of wild type 1 poliovirus in Gaza district in 2002.

Advanced environmental surveillance and molecular analyses indicate separate importations rather than endemic circulation of wild type 1 poliovirus in Gaza district in 2002. Research Abstract Details 

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Advanced environmental surveillance and molecular analyses indicate separate importations rather than endemic circulation of wild type 1 poliovirus in Gaza district in 2002. Abstract Text:

Y Manor,S Blomqvist,D Sofer,J Alfandari,T Halmut,B Abramovitz,E Mendelson,L M Shulman,Y Manor,S Blomqvist,D Sofer,J Alfandari,T Halmut,B Abramovitz,E Mendelson,L M Shulman,

An improved sewage surveillance algorithm (sample acquisition, processing, and molecular analysis) for wild and vaccine-derived polioviruses was developed and validated. It was based on plaque isolation with sensitive and high-throughput methods. The molecular analysis included sequencing; a comparison of the type, rate, and distribution of nucleotide substitutions with a profile for outbreaks evolving from a single progenitor; and phylogenetic analysis for relative similarity. The analyses revealed that two environmental wild type 1 isolates from the Gaza district in 2002 were imported separately, most likely from Egyptian southern governorates, and were not linked by endemic circulation. These findings illustrate the continuous spreading potential of wild-type poliovirus and underscore the value of extensive environmental surveillance employing advanced molecular analysis to monitor wild poliovirus in poliomyelitis-free regions.

Advanced environmental surveillance and molecular analyses indicate separate importations rather than endemic circulation of wild type 1 poliovirus in Gaza district in 2002. Publishing Authors By Initials

Y Manor,S Blomqvist,D Sofer,J Alfandari,T Halmut,B Abramovitz,E Mendelson,LM Shulman,Y Manor,S Blomqvist,D Sofer,J Alfandari,T Halmut,B Abramovitz,E Mendelson,LM Shulman,

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Advanced environmental surveillance and molecular analyses indicate separate importations rather than endemic circulation of wild type 1 poliovirus in Gaza district in 2002. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Applied and environmental microbiology

VOLUME: 73

Page Numbers: 5954-8

Journal Abbreviation: Appl. Environ. Microbiol.

ISSN: 0099-2240

DAY: 27

MONTH: 07

YEAR: 2007

Advanced environmental surveillance and molecular analyses indicate separate importations rather than endemic circulation of wild type 1 poliovirus in Gaza district in 2002. Information

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LANGUAGE: eng

NlmUniqueID: 7605801

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Grant and Affiliation Information for Advanced environmental surveillance and molecular analyses indicate separate importations rather than endemic circulation of wild type 1 poliovirus in Gaza district in 2002.

AFFILIATION: Public Health Services, Israel Ministry of Health, Central Virology Laboratory, Chaim Sheba Medical Center, Tel Hashomer, Israel.

Country: United States

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MEDLINETA: Appl Environ Microbiol

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ACCESSION NUMBER: AY923837

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