Adjuvant effects of plasmid-generated hairpin RNA molecules on DNA vaccination.

Adjuvant effects of plasmid-generated hairpin RNA molecules on DNA vaccination. Research Abstract Details 

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Adjuvant effects of plasmid-generated hairpin RNA molecules on DNA vaccination. Abstract Text:

Dingfeng Li,Yong Liu,Yuwei Zhang,Jianqing Xu,Kunxue Hong,Maosheng Sun,Yiming Shao,

Cellular recognition of double-stranded RNA and subsequent antiviral molecular events are important components of host defense, which are responsible for initiating innate immune responses to infection. Here we showed that hairpin RNA molecules with various stem lengths, which were transcribed from antigen-encoding plasmids had profound effects on the host cell apoptosis, foreign gene expression as well as the antigen-specific immune responses elicited by DNA vaccination. The plasmid generating the short-stem (40bp) RNA molecule showed slight effects on cell apoptosis and reporter gene expression level but stimulated significantly enhanced antigen-specific cellular immune responses. Although the DNA construct encoding the long-stem (750bp) RNA induced vigrous cell apoptosis, no significant improvement in cell-medicated immune responses was observed when mice were immunized with DNA vaccines encoding the long-stem RNA. In addition, our data also showed that none of DNA vaccine constructs carrying hairpin RNA cassettes enhanced antigen-specific humoral immune responses. This study introduced a novel approach for improving plasmid vector design and showed that the DNA vectors generating hairpin RNA in vivo have a great potential in vaccination and immunotherapy against infectious and malignant diseases. .

Adjuvant effects of plasmid-generated hairpin RNA molecules on DNA vaccination. Publishing Authors By Initials

D Li,Y Liu,Y Zhang,J Xu,K Hong,M Sun,Y Shao,

For similar proteins: recombinant proteins: vaccines, synthetic: vaccines, dna research abstracts see: proteins: recombinant proteins: vaccines, synthetic: vaccines, dna research

PUBMED ID PMID:

MEDLINE DATE:

Adjuvant effects of plasmid-generated hairpin RNA molecules on DNA vaccination. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Vaccine

VOLUME: 25

Page Numbers: 6992-7000

Journal Abbreviation: Vaccine

ISSN: 0264-410X

DAY: 16

MONTH: 07

YEAR: 2007

Adjuvant effects of plasmid-generated hairpin RNA molecules on DNA vaccination. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8406899

Adjuvant effects of plasmid-generated hairpin RNA molecules on DNA vaccination. Keywords Mesh Terms:

KEYWORDS: Vaccines, DNA

MESH TERMS: immunology

Chemical & Substance for Abstract: Adjuvant effects of plasmid-generated hairpin RNA molecules on DNA vaccination. Information

Substance Name: Luciferases

Registry Number: EC 1.13.12.-

Grant and Affiliation Information for Adjuvant effects of plasmid-generated hairpin RNA molecules on DNA vaccination.

AFFILIATION: Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Kunming 650118, China.

Country: Netherlands

AGENCY: United States NIAID

GRANT: 1 U19 AI51915-02

ACRONYM: AI

MEDLINETA: Vaccine

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