Adjacent residues in the E1 initiator beta-hairpin define different roles of the beta-hairpin in Ori melting, helicase loading, and helicase activity.

Adjacent residues in the E1 initiator beta-hairpin define different roles of the beta-hairpin in Ori melting, helicase loading, and helicase activity. Research Abstract Details 

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Adjacent residues in the E1 initiator beta-hairpin define different roles of the beta-hairpin in Ori melting, helicase loading, and helicase activity. Abstract Text:

Xiaofei Liu,Stephen Schuck,Arne Stenlund,

We have analyzed two residues in the helicase domain of the E1 initiator protein. These residues are part of a highly conserved structural motif, the beta-hairpin, which is present in the helicase domain of all papovavirus initiator proteins. These proteins are unique in their ability to transition from local template melting activity to unwinding. We demonstrate that the beta-hairpin has two functions. First, it is the tool used by the E1 double trimer (DT) to pry open and melt double-stranded DNA. Second, it is required for the unwinding activity of the hexameric E1 helicase. The fact that the same structural element, but not the same residues, contacts both dsDNA in the DT for melting and ssDNA in the double hexamer (DH) for helicase activity provides a link between local origin melting and DNA helicase activity and suggests how the transition between these two states comes about.

Adjacent residues in the E1 initiator beta-hairpin define different roles of the beta-hairpin in Ori melting, helicase loading, and helicase activity. Publishing Authors By Initials

X Liu,S Schuck,A Stenlund,

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

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Adjacent residues in the E1 initiator beta-hairpin define different roles of the beta-hairpin in Ori melting, helicase loading, and helicase activity. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular cell

VOLUME: 25

Page Numbers: 825-37

Journal Abbreviation: Mol. Cell

ISSN: 1097-2765

DAY: 23

MONTH: Mar

YEAR: 2007

Adjacent residues in the E1 initiator beta-hairpin define different roles of the beta-hairpin in Ori melting, helicase loading, and helicase activity. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9802571

Adjacent residues in the E1 initiator beta-hairpin define different roles of the beta-hairpin in Ori melting, helicase loading, and helicase activity. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Adjacent residues in the E1 initiator beta-hairpin define different roles of the beta-hairpin in Ori melting, helicase loading, and helicase activity. Information

Substance Name: DNA Helicases

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Adjacent residues in the E1 initiator beta-hairpin define different roles of the beta-hairpin in Ori melting, helicase loading, and helicase activity.

AFFILIATION: Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA 13106

ACRONYM: CA

MEDLINETA: Mol Cell

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