Adhesion of hematopoietic progenitor cells to human mesenchymal stem cells as a model for cell-cell interaction.

Adhesion of hematopoietic progenitor cells to human mesenchymal stem cells as a model for cell-cell interaction. Research Abstract Details 

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Adhesion of hematopoietic progenitor cells to human mesenchymal stem cells as a model for cell-cell interaction. Abstract Text:

Wolfgang Wagner,Frederik Wein,Christoph Roderburg,Rainer Saffrich,Anne Faber,Ulf Krause,Mario Schubert,Vladimir Benes,Volker Eckstein,Holger Maul,Anthony D Ho,

OBJECTIVE: The significant role of direct contact between hematopoietic progenitor cells (HPC) and the cellular microenvironment for maintaining "stemness" has been demonstrated. Human mesenchymal stem cell (MSC) feeder layers represent a surrogate model for this interaction. Specific adhesion molecules are responsible for this cell-cell contact. METHODS: To define cell-cell contact between HPC and MSC, we have studied adhesive interaction of various fractions of HPC by using a novel assay based on gravitational force upon inversion. Adherent and nonadherent cells were separated and further analyzed with regard to gene expression and long-term hematopoietic culture initiating cell (LTC-IC) frequency. RESULTS: HPC subsets with higher self-renewing capacity demonstrated significantly higher adherence to human MSC (CD34(+) vs CD34(-), CD34(+)/CD38(-) vs CD34(+)/CD38(+), slow dividing fraction vs fast dividing fraction). LTC-IC frequency was significantly higher in the adherent fraction than in the nonadherent fraction. Furthermore, genes coding for adhesion proteins and extracellular matrix were higher expressed in the adherent subsets of CD34(+) cells (fibronectin 1, cadherin 11, vascular cell adhesion molecule-1, connexin 43, integrin beta-like 1, and TGFBI). CONCLUSION: In this study we have demonstrated that primitive subsets of HPC have higher affinity to human MSC. The essential role of specific junction proteins for stabilization of cell-cell contact is indicated by their significant higher expression.

Adhesion of hematopoietic progenitor cells to human mesenchymal stem cells as a model for cell-cell interaction. Publishing Authors By Initials

W Wagner,F Wein,C Roderburg,R Saffrich,A Faber,U Krause,M Schubert,V Benes,V Eckstein,H Maul,AD Ho,

For similar membrane glycoproteins: cell adhesion molecules: vascular cell adhesion molecule-1 research abstracts see: membrane glycoproteins: cell adhesion molecules: vascular cell adhesion molecule-1 research

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Adhesion of hematopoietic progenitor cells to human mesenchymal stem cells as a model for cell-cell interaction. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Experimental hematology

VOLUME: 35

Page Numbers: 314-25

Journal Abbreviation: Exp. Hematol.

ISSN: 0301-472X

DAY: 30

MONTH: Feb

YEAR: 2007

Adhesion of hematopoietic progenitor cells to human mesenchymal stem cells as a model for cell-cell interaction. Information

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LANGUAGE: eng

NlmUniqueID: 402313

Adhesion of hematopoietic progenitor cells to human mesenchymal stem cells as a model for cell-cell interaction. Keywords Mesh Terms:

KEYWORDS: Vascular Cell Adhesion Molecule-1

MESH TERMS: immunology

Chemical & Substance for Abstract: Adhesion of hematopoietic progenitor cells to human mesenchymal stem cells as a model for cell-cell interaction. Information

Substance Name: osteoblast cadherin

Registry Number: 156621-71-5

Grant and Affiliation Information for Adhesion of hematopoietic progenitor cells to human mesenchymal stem cells as a model for cell-cell interaction.

AFFILIATION: Department of Medicine V, University of Heidelberg, Heidelberg, Germany.

Country: Netherlands

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MEDLINETA: Exp Hematol

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