Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord.

Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord. Abstract Text:

Masao Koda,Takahito Kamada,Masayuki Hashimoto,Masazumi Murakami,Hiroshi Shirasawa,Seiichiro Sakao,Hidetoshi Ino,Katsunori Yoshinaga,Shuhei Koshizuka,Hideshige Moriya,Masashi Yamazaki,Masao Koda,Takahito Kamada,Masayuki Hashimoto,Masazumi Murakami,Hiroshi Shirasawa,Seiichiro Sakao,Hidetoshi Ino,Katsunori Yoshinaga,Shuhei Koshizuka,Hideshige Moriya,Masashi Yamazaki,Masao Koda,Takahito Kamada,Masayuki Hashimoto,Masazumi Murakami,Hiroshi Shirasawa,Seiichiro Sakao,Hidetoshi Ino,Katsunori Yoshinaga,Shuhei Koshizuka,Hideshige Moriya,Masashi Yamazaki,

The aim of this study was to evaluate the efficacy in adult rat completely transected spinal cord of adenovirus vector-mediated brain-derived neurotrophic factor (BDNF) ex vivo gene transfer to bone marrow stromal cells (BMSC). BMSC were infected with adenovirus vectors carrying beta-galactosidase (AxCALacZ) or BDNF (AxCABDNF) genes. The T8 segment of spinal cord was removed and replaced by graft containing Matrigel alone (MG group) or Matrigel and BMSC infected by AxCALacZ (BMSC-LacZ group) or AxCABDNF (BMSC-BDNF group). Axons in the graft were evaluated by immunohistochemistry and functional recovery was assessed with BBB locomotor scale. In the BMSC-BDNF group, the number of fibers positive for growth associated protein-43, tyrosine hydroxylase, and calcitonin gene-related peptide was significantly larger than numbers found for the MG and BMSC-LacZ groups. Rats from BMSC-BDNF and BMSC-LacZ groups showed significant recovery of hind limb function compared with MG rats; however, there was no significant difference between groups in degree of functional recovery. These findings demonstrate that adenovirus vector-mediated ex vivo gene transfer of BDNF enhances the capacity of BMSC to promote axonal regeneration in this completely transected spinal cord model; however, BDNF failed to enhance hind limb functional recovery. Further investigation is needed to establish an optimal combination of cell therapy and neurotrophin gene transfer for cases of spinal cord injury.

Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord. Publishing Authors By Initials

M Koda,T Kamada,M Hashimoto,M Murakami,H Shirasawa,S Sakao,H Ino,K Yoshinaga,S Koshizuka,H Moriya,M Yamazaki,M Koda,T Kamada,M Hashimoto,M Murakami,H Shirasawa,S Sakao,H Ino,K Yoshinaga,S Koshizuka,H Moriya,M Yamazaki,M Koda,T Kamada,M Hashimoto,M Murakami,H Shirasawa,S Sakao,H Ino,K Yoshinaga,S Koshizuka,H Moriya,M Yamazaki,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: European spine journal : official publication of t

VOLUME: 16

Page Numbers: 2206-14

Journal Abbreviation:

ISSN: 0940-6719

DAY: 21

MONTH: 09

YEAR: 2007

Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9301980

Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord.

AFFILIATION: Department of Orthopaedic Surgery, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan, m-koda@bb.em-net.ne.jp.

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Eur Spine J

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord Related Publications

• A novel point mutation in the gene encoding capillary morphogenesis protein 2 in a Japanese patient with juvenile hyaline fibromatosis.
• Adenovirus vector-mediated ex vivo gene transfer of brain-derived neurotrophic factor to bone marrow stromal cells promotes axonal regeneration after transplantation in completely transected adult rat spinal cord.
• Autonomous regulation of sex-specific developmental programming in mouse fetal germ cells.
• Characteristics of congestive heart failure accompanied by atrial fibrillation with special reference to tachycardia-induced cardiomyopathy.
• Characterization of Aspergillus oryzae aspartyl aminopeptidase expressed in Escherichia coli.
• Current opinion: safety evaluation of drug metabolites in development of pharmaceuticals.
• Direct observation of reduction of met- and ferrylmyoglobins in the hemoglobin-free perfused rat heart.
• Effects of 2,4-substituents of deuterohemin upon peroxidase functions. I. Preparation and some properties of artificial enzymes.
• Expressions and mechanical functions of alpha1-adrenoceptor subtypes in hamster ureter.
• Formate dehydrogenase. Subunit and mechanism of inhibition by cyanide and azide.
• Heparin-induced thrombocytopenia, anomalous laboratory findings lead to the correct diagnosis.
• Hypersensitivity pneumonitis caused by Penicillium citrinum, not enoki spores.
• Individual differences and the chemical senses.
• Involvement of human cytochrome P450 2B6 in the omega- and 4-hydroxylation of the anesthetic agent propofol.
• One-electron and two-electron reductions of acceptors by xanthine oxidase and xanthine dehydrogenase.
• Postmortem diagnosis of Fabry disease with acromegaly and a unique vasculopathy.
• Prediction of cardiac events in patients with dilated cardiomyopathy using 123I-BMIPP and 201Tl myocardial scintigraphy.
• Preparation of bovine milk xanthine oxidase as a dehydrogenase form.
• Purification and some properties of formate dehydrogenase.
• Quality improvement of environmental secondary electron detector signal using helium gas in variable pressure scanning electron microscopy.
• Quantitative messenger RNA expression of Toll-like receptors and interferon-alpha1 in gingivitis and periodontitis.
• Rapidly progressive fatal pneumococcal sepsis in adults: a report of two cases.
• Reactions of the oxyform of horseradish peroxidase.
• Relationship between periodontal infections and systemic disease.
• Steady-state kinetics of the catalase reaction in the presence of cyanide.
• Synthesis and inhibitory activity of 4-alkynyl and 4-alkenylquinazolines: identification of new scaffolds for potent EGFR tyrosine kinase inhibitors.
• The 2-electron reduction of sperm whale ferryl myoglobin by ethanol.
• The effect of K+ concentration on the energy metabolism in perfused rat heart.
• The reaction of carbon monoxide with myoglobin in solution, in an amorphous state, and in crystals.
• Transient trimethylaminuria related to menstruation.